Department of Obstetrics and Gynecology, The First Affiliate Hospital of Jinan University, Jinan University, Guangzhou 510630, China.
International Joint Laboratory for Embryonic Development & Prenatal Medicine, Division of Histology and Embryology, Medical College, Jinan University, Guangzhou 510632, China; Key Laboratory for Regenerative Medicine of the Ministry of Education, Jinan University, Guangzhou 510632, China.
J Reprod Immunol. 2023 Mar;156:103815. doi: 10.1016/j.jri.2023.103815. Epub 2023 Jan 23.
Animal models are indispensable tools in studying the mechanisms underlying the diseases. Rat models with reduced uterine perfusion pressure (RUPP) were able to mimic the pathophysiological traits of placental ischemia and hypoxia in preeclampsia (PE). However, ischemic injury can lead to a cascade of damage to lower limb ischemia in RUPP. Therefore, the aim of our study was to compare three modified surgical procedures of reducing uteroplacental perfusion pressure, and to provide a reference for the recognition of different PE phenotypes in the future.
To establish a specific uteroplacental malperfusion model of PE in rats, we bilaterally ligated uterine vessels (UU), ovarian vessels distal to ovarian branches (OO), or both (sRUPP) at 13.5 days post coitum. 21 Sprague-Dawley rats in total were used and were divided into four groups: Sham (n = 4), UU (n = 6), OO (n = 5) and sRUPP (n = 8).
The results showed that the OO and sRUPP groups could successfully mimic the phenotypes of PE while UU group not. Then, autophagy, apoptosis, and synthesis of unsaturated fatty acids were increased in both the OO and sRUPP groups compared with the Sham group, while inflammation were not statistically different.
The OO and sRUPP groups could successfully establish the rat model of PE while the UU group not. Notably, between the OO and sRUPP groups, the OO group has a higher fetal survival rate and might be more suitable for studying fetal-related questions, while the sRUPP group has a heavier phenotypic profile and is more suitable for studying maternal phenotypes related to PE.
动物模型是研究疾病发病机制不可或缺的工具。通过减少子宫灌注压（RUPP）的大鼠模型能够模拟子痫前期（PE）中胎盘缺血和缺氧的病理生理特征。然而，缺血性损伤会导致 RUPP 下肢缺血的级联损伤。因此，本研究旨在比较三种降低子宫胎盘灌注压的改良手术方法，为未来识别不同的 PE 表型提供参考。
为了在大鼠中建立特定的子痫前期子宫胎盘灌注不良模型，我们在妊娠第 13.5 天对子宫血管（UU）和卵巢分支远端的卵巢血管（OO）或两者（sRUPP）进行双侧结扎。总共使用了 21 只 Sprague-Dawley 大鼠，并将其分为四组：假手术组（n=4）、UU 组（n=6）、OO 组（n=5）和 sRUPP 组（n=8）。
结果表明，OO 组和 sRUPP 组能够成功模拟 PE 表型，而 UU 组则不能。然后，与假手术组相比，OO 组和 sRUPP 组的自噬、凋亡和不饱和脂肪酸合成增加，而炎症无统计学差异。
OO 组和 sRUPP 组能够成功建立 PE 大鼠模型，而 UU 组则不能。值得注意的是，在 OO 组和 sRUPP 组之间，OO 组具有更高的胎儿存活率，可能更适合研究与胎儿相关的问题，而 sRUPP 组具有更重的表型特征，更适合研究与 PE 相关的母体表型。
