IRTA, Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Campus UAB, 08193 Bellaterra, Barcelona, Spain.
Biotechnology of Natural Products, Technische Universität München, Liesel-Beckmann-Str. 1, 85354 Freising, Germany.
Plant Sci. 2016 Jan;242:151-168. doi: 10.1016/j.plantsci.2015.07.019. Epub 2015 Aug 19.
Over the last few years, diploid strawberry (Fragaria vesca) has been recognized as a model species for applied research of cultivated strawberry (Fragaria × ananassa) that is one of the most economically important crops. Berries, particularly strawberries, are known for their high antioxidant capacity due to a high concentration of (poly) phenolic compounds. Studies have already characterized the phenolic composition of fruits from sets of cultivated strawberries but the quantification of phenolics in a Fragaria mapping population has not been reported, yet. The metabolite profiling of a F. vesca near isogenic line (NIL) collection by LC-MS allowed the unambiguous identification of 22 (poly)-phenols, including anthocyanins, flavonols, flavan-3-ols, flavanones, hydroxycinnamic acid derivatives, and ellagic acid in the diploid strawberry fruit. The variability in the collection revealed that the genetic factor was more decisive than the environmental factor for the accumulation of 18 of the 24 compounds. Genotyping the NIL collection with the Axiom® IStraw90® SNPs array, we were able to map 76 stable QTLs controlling accumulation of the (poly)-phenolic compounds. They provide a powerful new tool to characterise candidate genes to increase the antioxidant capacity of fruits and produce healthier strawberries for consumers.
在过去的几年中，二倍体草莓（Fragaria vesca）已被认为是栽培草莓（Fragaria ×ananassa）应用研究的模式物种，而栽培草莓是最具经济重要性的作物之一。浆果，尤其是草莓，由于富含（多）酚类化合物而具有高抗氧化能力。已经对一系列栽培草莓果实的酚类成分进行了研究，但尚未报道在 Fragaria 作图群体中测定酚类物质的定量。通过 LC-MS 对 F. vesca 近等基因系（NIL）集合进行的代谢物分析可明确鉴定出 22 种（多）酚类物质，包括二倍体草莓果实中的花色苷、类黄酮、黄烷醇、黄酮醇、羟基肉桂酸衍生物和鞣花酸。该集合的变异性表明，遗传因素比环境因素对 24 种化合物中的 18 种的积累更为决定性。通过使用 Axiom®IStraw90®SNP 芯片对 NIL 集合进行基因分型，我们能够绘制 76 个控制（多）酚类化合物积累的稳定 QTL。它们为鉴定候选基因提供了一种强大的新工具，以提高水果的抗氧化能力，为消费者生产更健康的草莓。
