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采用 UHPLC-HRMS(n.) 对两个二倍体草莓(Fragaria vesca)自交系的多酚进行分析。

Profiling polyphenols of two diploid strawberry (Fragaria vesca) inbred lines using UHPLC-HRMS(n.).

机构信息

U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Food Composition and Methods Development Laboratory, Beltsville, MD 20705, United States.

出版信息

Food Chem. 2014 Mar 1;146:289-98. doi: 10.1016/j.foodchem.2013.08.089. Epub 2013 Sep 12.

DOI:10.1016/j.foodchem.2013.08.089
PMID:24176345
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3902803/
Abstract

Phenolic compounds in the fruits of two diploid strawberries (Fragaria vesca f. semperflorens) inbred lines-Ruegen F7-4 (a red-fruited genotype) and YW5AF7 (a yellow-fruited genotype) were characterised using ultra-high-performance liquid chromatography coupled with tandem high-resolution mass spectrometry (UHPLC-HRMS(n)). The changes of anthocyanin composition during fruit development and between Ruegen F7-4 and YW5AF7 were studied. About 67 phenolic compounds, including taxifolin 3-O-arabinoside, glycosides of quercetin, kaempferol, cyanidin, pelargonidin, peonidin, ellagic acid derivatives, and other flavonols were identified in these two inbred lines. Compared to the regular octoploid strawberry, unique phenolic compounds were found in F. vesca fruits, such as taxifolin 3-O-arabinoside (both) and peonidin 3-O-malonylglucoside (Ruegen F7-4). The results provide the basis for comparative analysis of polyphenolic compounds in yellow and red diploid strawberries, as well as with the cultivated octoploid strawberries.

摘要

利用超高效液相色谱-串联高分辨质谱联用技术(UHPLC-HRMS(n))对两个二倍体草莓(Fragaria vesca f. semperflorens)自交系果实中的酚类化合物进行了表征,Ruegen F7-4(红色果实基因型)和 YW5AF7(黄色果实基因型)。研究了果实发育过程中花色苷组成的变化以及 Ruegen F7-4 和 YW5AF7 之间的差异。在这两个自交系中鉴定出了约 67 种酚类化合物,包括杨梅素 3-O-阿拉伯糖苷、槲皮素、山奈酚、矢车菊素、天竺葵素、芍药素、鞣花酸衍生物和其他类黄酮的糖苷。与常规的八倍体草莓相比,在 F. vesca 果实中发现了一些独特的酚类化合物,如杨梅素 3-O-阿拉伯糖苷(两者)和芍药素 3-O-丙二酰基葡萄糖苷(Ruegen F7-4)。该结果为分析黄色和红色二倍体草莓以及栽培的八倍体草莓中多酚类化合物提供了依据。

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