College of Agronomy, Northwest A&F University, Yangling, 712100 Shaanxi, People's Republic of China; State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000 Henan, People's Republic of China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000 Henan, People's Republic of China.
Plant Sci. 2016 Jan;242:178-186. doi: 10.1016/j.plantsci.2015.05.001. Epub 2015 May 11.
In Arabidopsis flowering pathway, MADS-box genes encode transcription factors, with their structures and functions highly conserved in many species. In our study, two MADS-box genes GhSOC1 and GhMADS42 (Gossypium hirsutum L.) were cloned from upland cotton CCRI36 and transformed into Arabidopsis. GhSOC1 was additionally transformed into upland cotton. Comparative analysis demonstrated sequence conservation between GhSOC1 and GhMADS42 and genes of other plant species. Tissue-specific expression analysis of GhSOC1 and GhMADS42 revealed spatiotemporal expression patterns involving high transcript levels in leaves, shoot apical buds, and flowers. In addition, overexpression of both GhSOC1 and GhMADS42 in Arabidopsis accelerated flowering, with GhMADS42 transgenic plants showing abnormal floral organ phenotypes. Overexpression of GhSOC1 in upland cotton also produced variations in floral organs. Furthermore, chromatin immunoprecipitation assay demonstrated that GhSOC1 could regulate GhMADS41 and GhMADS42, but not FLOWERING LOCUS T, by directly binding to the genes promoter. Finally, yeast two-hybrid and bimolecular fluorescence complementation approaches were undertaken to better understand the interaction of GhSOC1 and other MADS-box factors. These experiments showed that GhSOC1 can interact with APETALA1/FRUITFULL-like proteins in cotton.
在拟南芥开花途径中，MADS-box 基因编码转录因子，其结构和功能在许多物种中高度保守。在我们的研究中，从陆地棉 CCRI36 中克隆了两个 MADS-box 基因 GhSOC1 和 GhMADS42（Gossypium hirsutum L.），并将其转化为拟南芥。GhSOC1 还被转化到陆地棉中。序列比较分析表明 GhSOC1 和 GhMADS42 与其他植物物种的基因具有序列保守性。GhSOC1 和 GhMADS42 的组织特异性表达分析显示了涉及叶片、茎尖芽和花中高转录水平的时空表达模式。此外，GhSOC1 和 GhMADS42 在拟南芥中的过表达均加速了开花，GhMADS42 转基因植物表现出异常的花器官表型。GhSOC1 在陆地棉中的过表达也导致花器官发生变异。此外，染色质免疫沉淀分析表明，GhSOC1 可以通过直接结合基因启动子来调节 GhMADS41 和 GhMADS42，但不能调节 FLOWERING LOCUS T。最后，进行了酵母双杂交和双分子荧光互补实验，以更好地了解 GhSOC1 和其他 MADS-box 因子的相互作用。这些实验表明，GhSOC1 可以与棉花中的 APETALA1/FRUITFULL 样蛋白相互作用。
