Key Laboratory of Cotton Genetic Improvement of Ministry of Agriculture, The Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, People's Republic of China.
Plant Cell Rep. 2013 Nov;32(11):1675-86. doi: 10.1007/s00299-013-1479-1. Epub 2013 Jul 27.
GhLFY was cloned from G. hirsutum L. Its expression, subcellular localization, and function were analyzed, as well as the in vivo regulation of GhLFY by the MADS-box protein SOC1 (GhSOC1).
Flowering is a very important phase during which plants produce the organs for sexual reproduction. The FLORICAULA/LEAFY (FLO/LFY) homologs play a major role in the initiation of flowering. To understand the mechanism of the transition from the vegetative to reproductive phases in Upland cotton (Gossypium hirsutum L.), we isolated a candidate LFY gene from G. hirsutum L. (GhLFY) that showed a high degree of similarity to other plant homologs of FLO/LFY. qPCR analysis showed that GhLFY was highly expressed in the shoot apex, with substantial upregulation at the third true leaf expansion stage during floral bud differentiation. Subcellular localization studies revealed GhLFY localization in the nucleus. Ectopic expression of the GhLFY coding region in Arabidopsis resulted in early flowering. The expression of the GhLFY coding region under the control of the 35S promoter complemented the lfy-5 mutation in transgenic Arabidopsis lfy-5 mutant plants. Furthermore, a chromatin immunoprecipitation assay revealed that GhLFY may function downstream of GhSOC1 during the initiation of flowering in G. hirsutum L. GhLFY was likely to be regulated by GhSOC1, which binds to the LFY promoter in Arabidopsis. These results suggest that GhLFY is a FLO/LFY ortholog that may be involved in controlling flowering time and floral development.
GhLFY 从 G. hirsutum L. 中克隆而来。分析了其表达、亚细胞定位和功能，以及 MADS 框蛋白 SOC1（GhSOC1）对 GhLFY 的体内调控。
开花是植物产生有性繁殖器官的非常重要的阶段。FLORICAULA/LEAFY（FLO/LFY）同源物在开花启动中起主要作用。为了了解陆地棉（Gossypium hirsutum L.）从营养生长到生殖生长的转变机制，我们从 G. hirsutum L. 中分离出一个候选 LFY 基因（GhLFY），它与 FLO/LFY 的其他植物同源物具有高度相似性。qPCR 分析显示，GhLFY 在茎尖中高度表达，在花芽分化过程中第三真叶展开阶段大量上调。亚细胞定位研究表明 GhLFY 定位于细胞核。在拟南芥中异位表达 GhLFY 编码区导致早花。在转基因拟南芥 lfy-5 突变体植物中，35S 启动子控制下的 GhLFY 编码区的表达补充了 lfy-5 突变。此外，染色质免疫沉淀分析表明，GhLFY 可能在 G. hirsutum L. 开花启动过程中位于 GhSOC1 下游。GhLFY 可能受 GhSOC1 调控，GhSOC1 在拟南芥中与 LFY 启动子结合。这些结果表明，GhLFY 是 FLO/LFY 的同源物，可能参与控制开花时间和花发育。
