Tian Jilai, Yang Fang, Cui Huating, Zhou Ying, Ruan Xiaobo, Gu Ning
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Sciences & Medical Engineering, Southeast University , Nanjing 210096, China.
Collaborative Innovation Center of Suzhou Nano-Science and Technology, Suzhou Key Laboratory of Biomaterials and Technologies , Suzhou 215123, China.
ACS Appl Mater Interfaces. 2015 Dec 9;7(48):26579-84. doi: 10.1021/acsami.5b07778. Epub 2015 Nov 24.
Nanobubbles with a size less than 1 μm could make a promising application in ultrasound molecular imaging and drug delivery. However, the fabrication of stable gas encapsulation nanobubbles is still challenging. In this study, a novel method for preparation of lipid- encapsulated nanobubbles was reported. The dispersed phospholipid molecules in the prefabricated free nanobubbles solution can be assembled to form controllable stable lipid encapsulation gas-filled ultrasound-sensitive liposome (GU-Liposome). The optimized preparation parameters and formation mechanism of GU-Liposome were investigated in detail. Results showed that this type of GU-Liposome had mean diameter of 194.4 ± 6.6 nm and zeta potential of -25.2 ± 1.9 mV with layer by layer self-assembled lipid structure. The acoustic imaging analysis in vitro indicated that ultrasound imaging enhancement could be acquired by both perfusion imaging and accumulation imaging. The imaging enhancement level and duration time was related with the ratios of lipid to gas in the GU-Liposome structure. All in all, by this novel and controllable nanobubble construction technique, it will broaden the future theranostic applications of nanobubbles.
尺寸小于1μm的纳米气泡在超声分子成像和药物递送方面具有广阔的应用前景。然而，制备稳定的气体包封纳米气泡仍然具有挑战性。在本研究中，报道了一种制备脂质包封纳米气泡的新方法。预制的游离纳米气泡溶液中分散的磷脂分子可以组装形成可控的稳定脂质包封气体填充超声敏感脂质体（GU-脂质体）。详细研究了GU-脂质体的优化制备参数和形成机制。结果表明，这种类型的GU-脂质体具有194.4±6.6nm的平均直径和-25.2±1.9mV的zeta电位，具有逐层自组装脂质结构。体外声学成像分析表明，灌注成像和积聚成像均可获得超声成像增强。成像增强水平和持续时间与GU-脂质体结构中脂质与气体的比例有关。总之，通过这种新颖且可控的纳米气泡构建技术，将拓宽纳米气泡未来的诊疗应用。
