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基于超高效液相色谱-四极杆飞行时间质谱联用技术对桔梗中多种代谢产物的全面分析

Global Profiling of Various Metabolites in Platycodon grandiflorum by UPLC-QTOF/MS.

作者信息

Lee Jae Won, Ji Seung-Heon, Kim Geum-Soog, Song Kyung-Sik, Um Yurry, Kim Ok Tae, Lee Yi, Hong Chang Pyo, Shin Dong-Ho, Kim Chang-Kug, Lee Seung-Eun, Ahn Young-Sup, Lee Dae-Young

机构信息

Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea.

College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea.

出版信息

Int J Mol Sci. 2015 Nov 9;16(11):26786-96. doi: 10.3390/ijms161125993.

DOI:10.3390/ijms161125993
PMID:26569219
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4661852/
Abstract

In this study, a method of metabolite profiling based on UPLC-QTOF/MS was developed to analyze Platycodon grandiflorum. In the optimal UPLC, various metabolites, including major platycosides, were separated well in 15 min. The metabolite extraction protocols were also optimized by selecting a solvent for use in the study, the ratio of solvent to sample and sonication time. This method was used to profile two different parts of P. grandiflorum, i.e., the roots of P. grandiflorum (PR) and the stems and leaves of P. grandiflorum (PS), in the positive and negative ion modes. As a result, PR and PS showed qualitatively and quantitatively different metabolite profiles. Furthermore, their metabolite compositions differed according to individual plant samples. These results indicate that the UPLC-QTOF/MS-based profiling method is a good tool to analyze various metabolites in P. grandiflorum. This metabolomics approach can also be applied to evaluate the overall quality of P. grandiflorum, as well as to discriminate the cultivars for the medicinal plant industry.

摘要

在本研究中，开发了一种基于超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF/MS）的代谢物谱分析方法来分析桔梗。在优化的超高效液相色谱条件下，包括主要桔梗皂苷在内的各种代谢物在15分钟内得到了良好的分离。还通过选择研究中使用的溶剂、溶剂与样品的比例以及超声处理时间对代谢物提取方案进行了优化。该方法用于在正离子和负离子模式下分析桔梗的两个不同部位，即桔梗根（PR）和桔梗茎与叶（PS）。结果表明，PR和PS在代谢物谱的定性和定量方面均存在差异。此外，它们的代谢物组成因单个植物样品而异。这些结果表明，基于UPLC-QTOF/MS的谱分析方法是分析桔梗中各种代谢物的良好工具。这种代谢组学方法还可用于评估桔梗的整体质量，以及区分药用植物行业中的品种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e34d/4661852/8544d6945e57/ijms-16-25993-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e34d/4661852/b7f0ea7e714d/ijms-16-25993-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e34d/4661852/f96da15a47af/ijms-16-25993-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e34d/4661852/8544d6945e57/ijms-16-25993-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e34d/4661852/b7f0ea7e714d/ijms-16-25993-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e34d/4661852/f96da15a47af/ijms-16-25993-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e34d/4661852/8544d6945e57/ijms-16-25993-g003.jpg

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