Zorzi Alessandro R, Amstalden Eliane M I, Plepis Ana Maria G, Martins Virginia C A, Ferretti Mario, Antonioli Eliane, Duarte Adriana S S, Luzo Angela C M, Miranda João B
Department of Orthopedic Surgery, Faculty of Medical Sciences, Universidade de Campinas (UNICAMP), Campinas/SP 13083-887, Brazil.
Hospital Israelita Albert Einstein, São Paulo/SP 05652-900, Brazil.
Int J Mol Sci. 2015 Nov 9;16(11):26813-31. doi: 10.3390/ijms161125989.
Cell therapy is a promising approach to improve cartilage healing. Adipose tissue is an abundant and readily accessible cell source. Previous studies have demonstrated good cartilage repair results with adipose tissue mesenchymal stem cells in small animal experiments. This study aimed to examine these cells in a large animal model. Thirty knees of adult sheep were randomly allocated to three treatment groups: CELLS (scaffold seeded with human adipose tissue mesenchymal stem cells), SCAFFOLD (scaffold without cells), or EMPTY (untreated lesions). A partial thickness defect was created in the medial femoral condyle. After six months, the knees were examined according to an adaptation of the International Cartilage Repair Society (ICRS 1) score, in addition to a new Partial Thickness Model scale and the ICRS macroscopic score. All of the animals completed the follow-up period. The CELLS group presented with the highest ICRS 1 score (8.3 ± 3.1), followed by the SCAFFOLD group (5.6 ± 2.2) and the EMPTY group (5.2 ± 2.4) (p = 0.033). Other scores were not significantly different. These results suggest that human adipose tissue mesenchymal stem cells promoted satisfactory cartilage repair in the ovine model.
细胞疗法是一种改善软骨愈合的有前景的方法。脂肪组织是一种丰富且易于获取的细胞来源。先前的研究在小动物实验中已证明脂肪组织间充质干细胞具有良好的软骨修复效果。本研究旨在在大型动物模型中检验这些细胞。将30只成年绵羊的膝关节随机分为三个治疗组：细胞组（接种人脂肪组织间充质干细胞的支架）、支架组（无细胞的支架）或空白组（未治疗的损伤）。在内侧股骨髁制造一个部分厚度缺损。六个月后，除了一个新的部分厚度模型量表和国际软骨修复协会（ICRS 1）宏观评分外，还根据ICRS 1评分的改编版本对膝关节进行检查。所有动物均完成随访期。细胞组的ICRS 1评分最高（8.3±3.1），其次是支架组（5.6±2.2）和空白组（5.2±2.4）（p = 0.033）。其他评分无显著差异。这些结果表明人脂肪组织间充质干细胞在绵羊模型中促进了令人满意的软骨修复。
