Schneider Katherine, Wronka-Edwards Loni, Leggett-Embrey Melissa, Walker Eric, Sun Peifang, Ondov Brian, Wyman Travis H, Rosovitz M J, Bohn Sherry S, Burans James, Kochel Tadeusz
National Biodefense Analysis and Countermeasures Center (NBACC), Fort Detrick, MD 21702, USA.
Naval Medical Research Center, Silver Spring, MD 20910, USA.
Viruses. 2015 Nov 12;7(11):5875-88. doi: 10.3390/v7112912.
High consequence human pathogenic viruses must be handled at biosafety level 2, 3 or 4 and must be rendered non-infectious before they can be utilized for molecular or immunological applications at lower biosafety levels. Here we evaluate psoralen-inactivated Arena-, Bunya-, Corona-, Filo-, Flavi- and Orthomyxoviruses for their suitability as antigen in immunological processes and as template for reverse transcription PCR and sequencing. The method of virus inactivation using a psoralen molecule appears to have broad applicability to RNA viruses and to leave both the particle and RNA of the treated virus intact, while rendering the virus non-infectious.
高致病性人类病原病毒必须在生物安全2、3或4级条件下处理，并且在可用于较低生物安全级别的分子或免疫学应用之前，必须使其失去感染性。在此，我们评估了补骨脂素灭活的沙粒病毒、布尼亚病毒、冠状病毒、丝状病毒、黄病毒和正粘病毒作为免疫学过程中的抗原以及逆转录PCR和测序模板的适用性。使用补骨脂素分子进行病毒灭活的方法似乎对RNA病毒具有广泛适用性，并且能使处理后的病毒颗粒和RNA保持完整，同时使病毒失去感染性。
