Chen Chun, You Li-Jun, Abbasi Arshad Mehmood, Fu Xiong, Liu Rui Hai, Li Chao
College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.
Food Funct. 2016 Jan;7(1):530-9. doi: 10.1039/c5fo01114k.
The present study aimed to characterize and investigate the polysaccharide fractions of mulberry fruit, and their antioxidant and hypoglycemic activities. Mulberry fruit polysaccharides (MFP) were extracted by using hot water as the solvent and fractioned by using a DEAE-Sepharose fast-flow column. In total four eluents of water (MFP-1), 0.1 M NaCl (MFP-2), 0.2 M NaCl (MFP-3) and 0.3 M NaCl (MFP-4) were fractionated. Arabinose, galactose, glucose, xylose and mannose were the main components present in MFP-1, while MFP-2 and MFP-3 were composed of arabinose and galactose, and MFP-4 of arabinose, galactose and glucose. High-performance gel permeation chromatography (HP-GPC) analysis indicated a narrower molecular weight distribution in the MFP-1 fraction. Scanning electron microscopy (SEM) exhibited a smooth surface for MFP-1 and MFP-3 fractions, whereas MFP-2 and MFP-4 have pore openings and flocculent fibers on a smooth surface. Tertiary structure analyses indicated that none of the fractions had a triple-helical conformation. On the whole, MFP-3 and MFP-4 showed better antioxidant activity and inhibitory effects on α-amylase and α-glucosidase compared to MFP-1 and MFP-2. These results show significant potential for the antioxidant and hypoglycemic activities of MFP-3 and MFP-4 indicating the need for their further exploration as potential antidiabetic agents.
本研究旨在表征和研究桑椹果实的多糖组分及其抗氧化和降血糖活性。采用热水作为溶剂提取桑椹果实多糖（MFP），并用DEAE-琼脂糖快速流动柱进行分级分离。共分离出四种洗脱液，分别为水（MFP-1）、0.1 M NaCl（MFP-2）、0.2 M NaCl（MFP-3）和0.3 M NaCl（MFP-4）。阿拉伯糖、半乳糖、葡萄糖、木糖和甘露糖是MFP-1中的主要成分，而MFP-2和MFP-3由阿拉伯糖和半乳糖组成，MFP-4由阿拉伯糖、半乳糖和葡萄糖组成。高效凝胶渗透色谱（HP-GPC）分析表明MFP-1组分的分子量分布较窄。扫描电子显微镜（SEM）显示MFP-1和MFP-3组分表面光滑，而MFP-2和MFP-4在光滑表面上有孔隙和絮状纤维。三级结构分析表明，所有组分均无三螺旋构象。总体而言，与MFP-1和MFP-2相比，MFP-3和MFP-4表现出更好的抗氧化活性以及对α-淀粉酶和α-葡萄糖苷酶的抑制作用。这些结果表明MFP-3和MFP-4在抗氧化和降血糖活性方面具有显著潜力，表明有必要将其作为潜在的抗糖尿病药物进行进一步探索。
