Huang Yuanyuan, Xie Wen, Tang Ting, Chen Huaguo, Zhou Xin
Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, China.
Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, China.
Front Nutr. 2023 Apr 6;10:1125831. doi: 10.3389/fnut.2023.1125831. eCollection 2023.
The mulberry (), which is rich in many nutrients needed by the human body, serves as both food and medicine. Polysaccharides, which are considered to be important pharmacological components of mulberry, have received a lot of study for their structure and biological activity. In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared. According to the findings, MFP-III exhibited the best α-glucosidase and α-amylase inhibition, whereas MFP-IV had the strongest scavenging activity against DPPH and ABTS. Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques. The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods. According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides. This information can provide a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity.
桑椹富含人体所需的多种营养成分，兼具食用和药用价值。多糖被认为是桑椹的重要药理成分，其结构和生物活性受到了广泛研究。本研究采用不同提取方法提取了六种桑椹果实多糖（MFP），并对其理化结构、抗氧化和降血糖生物活性进行了研究和比较。结果表明，MFP-III对α-葡萄糖苷酶和α-淀粉酶的抑制作用最佳，而MFP-IV对DPPH和ABTS的清除活性最强。扫描电子显微镜（SEM）和高效液相色谱（HPLC）分析表明，不同提取技术对MFP的表观形态和单糖含量有显著影响。刚果红实验、傅里叶变换红外光谱（FT-IR）、核磁共振（NMR）、热重分析（TG）实验结果表明，不同提取方法之间MFP的官能团、糖苷键、三螺旋结构和热稳定性没有显著差异。根据上述研究，不同提取方法对桑椹多糖的化学组成和生物活性有不同影响。该信息可为选择合适的提取方法以获得具有理想生物活性的桑椹多糖提供科学依据。
