Tanaka K, O'Leary T J, Kafrawy A H
Department of Periodontics, Indiana University School of Dentistry, Indianapolis.
J Periodontol. 1989 Feb;60(2):81-3. doi: 10.1902/jop.1989.60.2.81.
Five extracted human teeth with clinically demonstrable calculus were used. One proximal surface of each tooth was partly scaled, leaving a small amount of calculus. The crown was then cut off at the cementoenamel junction and the scaled proximal surface was divided vertically into two segments using a thin separating disk. One segment was treated by rubbing cotton pellets soaked in citric acid (pH1) over the surface for three minutes and then immersing the segment in saline to stop the reaction. The other (control) segment was treated by rubbing cotton pellets soaked in saline over the surface for three minutes. When viewed under a scanning electron microscope, the control sections displayed scaling striations, considerable surface debris, and large numbers of bacteria at the borders of the residual calculus. Citric acid-treated specimens displayed little debris on cementum or residual calculus and virtually no bacteria at the junction between calculus and cementum. The surface morphology of the citric acid-treated calculus varied from layered-like to honeycomb.
使用了五颗临床上有明显牙结石的拔除人类牙齿。每颗牙齿的一个邻面部分刮治,留下少量牙结石。然后在牙骨质釉质界处切断牙冠,使用薄分离盘将刮治的邻面垂直分成两段。一段通过用浸泡在柠檬酸(pH1)中的棉球在表面摩擦三分钟,然后将该段浸入盐水中以停止反应来处理。另一段(对照)通过用浸泡在盐水中的棉球在表面摩擦三分钟来处理。在扫描电子显微镜下观察时,对照切片显示有刮治条纹、大量表面碎屑以及残留牙结石边缘有大量细菌。柠檬酸处理的标本在牙骨质或残留牙结石上几乎没有碎屑,在牙结石与牙骨质的交界处几乎没有细菌。柠檬酸处理的牙结石表面形态从层状到蜂窝状不等。