季铵化壳聚糖衍生物的合成、抗氧化和组织蛋白酶 D 抑制活性。

Synthesis, antioxidant and cathepsin D inhibition activity of quaternary ammonium chitosan derivatives.

机构信息

Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, PR China.

出版信息

Carbohydr Polym. 2016 Jan 20;136:884-91. doi: 10.1016/j.carbpol.2015.09.111. Epub 2015 Oct 9.

DOI:10.1016/j.carbpol.2015.09.111

PMID:26572425

Abstract

Two (2-hydroxypropyl) trimethyl ammonium and/or imidazole-based quaternary ammonium chitosan derivatives (NHT-chitosan and Im-OHT-chitosan) were synthesized by using nucleophilic substitution reaction. These two synthesized chitosan derivatives were characterized by Fourier transform infrared spectroscopy, NMR spectra, and UV-visible spectra. The applications as antioxidant agents and cathepsin D inhibitors were further investigated. Both of quaternary ammonium chitosan derivatives exhibited good antioxidant activity upon scavenging against hydroxyl radical and hydrogen peroxide as well as the lipid peroxidation inhibition in the linoleic acid emulsion system. They also exhibited good inhibition activity of cathepsin D protease. NHT-chitosan and Im-OHT-chitosan are potential the natural, healthy and safe preservatives in food industry.

摘要

两种（2-羟丙基）三甲基铵和/或咪唑基季铵化壳聚糖衍生物（NHT-壳聚糖和 Im-OHT-壳聚糖）通过亲核取代反应合成。这两种合成的壳聚糖衍生物通过傅里叶变换红外光谱、NMR 谱和紫外可见光谱进行了表征。进一步研究了它们作为抗氧化剂和组织蛋白酶 D 抑制剂的应用。两种季铵化壳聚糖衍生物在清除羟基自由基和过氧化氢以及抑制亚油酸乳液体系中的脂质过氧化方面均表现出良好的抗氧化活性。它们还对组织蛋白酶 D 蛋白酶表现出良好的抑制活性。NHT-壳聚糖和 Im-OHT-壳聚糖是食品工业中具有天然、健康和安全特性的潜在防腐剂。