Li Xiao-Peng, Zhai Shao-Lun, He Dong-Sheng, Guo Peng-Ju, Lv Dian-Hong, Wen Xiao-Hui, Luo Man-Lin, Chen Rui-Ai, Wei Wen-Kang
College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou, 510642, China.
Animal Disease Diagnostic Center, Institute of Animal Health, Guangdong Academy of Agricultural Sciences/Guangdong Key Labratory of Animal Disease Prevention, No. 21 Baishigang Street, Wushan Road, Tianhe District, Guangzhou, 510640, China.
Virus Genes. 2015 Dec;51(3):361-6. doi: 10.1007/s11262-015-1249-y. Epub 2015 Nov 14.
Since 2013, the second outbreak of peste des petits ruminants (PPR) caused by Peste des petits ruminants virus (PPRV) has spread over more than 20 provinces, municipalities, and autonomous regions in China, resulting in major economic losses for livestock industry. In 2014, we encountered a clinical PPR case on a goat farm in Guangdong province, southern China. The complete genome of this PPRV strain, named CH/GDDG/2014, was sequenced to determine its similarities and differences with other strains. The CH/GDDG/2014 genome comprised 15,954 nucleotides (six nucleotides more than classical PPRVs identified before 2013, but complying with the rule of six) with six open reading frames encoding nucleocapsid protein, phosphoprotein, matrix protein, fusion protein, hemagglutinin, and large polymerase protein, respectively. The whole-genome-based alignment analysis indicated that CH/GDDG/2014 had the most proximate consensus (99.8 %) to China/XJYL/2013 and the least consensus (87.2 %) to KN5/2011. The phylogenetic analysis showed that CH/GDDG/2014 was clustered in one branch (lineage IV) with other emerging strains during the second outbreak. This study is the first report describing the whole-genome sequence of PPRV in Guangdong province, southern China and also suggests the PPR outbreak may be closely related to illegal cross-regional importation of goats.
自2013年以来，由小反刍兽疫病毒（PPRV）引起的第二次小反刍兽疫（PPR）疫情已在中国20多个省、直辖市和自治区蔓延，给畜牧业造成了重大经济损失。2014年，我们在中国南方广东省的一个山羊养殖场遇到了一例PPR临床病例。对该PPRV毒株（命名为CH/GDDG/2014）的全基因组进行测序，以确定其与其他毒株的异同。CH/GDDG/2014基因组由15954个核苷酸组成（比2013年之前鉴定的经典PPRV多6个核苷酸，但符合六规则），有6个开放阅读框，分别编码核衣壳蛋白、磷蛋白、基质蛋白、融合蛋白、血凝素和大聚合酶蛋白。基于全基因组的比对分析表明，CH/GDDG/2014与China/XJYL/2013的一致性最高（99.8%），与KN5/2011的一致性最低（87.2%）。系统发育分析表明，CH/GDDG/2014与第二次疫情期间的其他新兴毒株聚集在一个分支（谱系IV）中。本研究首次报道了中国南方广东省PPRV的全基因组序列，也表明PPR疫情可能与非法跨区域进口山羊密切相关。
