Slabaugh M B, Roseman N A
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.
Proc Natl Acad Sci U S A. 1989 Jun;86(11):4152-5. doi: 10.1073/pnas.86.11.4152.
The retroviral protease-encoding region, PR, situated between the gag and pol genes, underwent gene duplication in the lineage now represented by simian retrovirus type 1; the sequence of the duplicated segment has diverged considerably from the present PR sequence [Power, M.D., Marx, P.A., Bryant, M.L., Gardner, M.B., Barr, P.J. & Luciw, P.A. (1986) Science 231, 1567-1572]. The PR-like duplicated gene segment was at some point translocated to a new site within the pol gene of a lentivirus (subsequent to the divergence of human immunodeficiency virus type 1), where it has been maintained. We have identified in the vaccinia virus genome a sequence that is homologous to the PR-like duplicated gene segment of both types of retrovirus in an open reading frame whose product is predicted to be a 16.2-kDa protein. The vaccinia PR-like gene is located in the HindIII F fragment, and its product displays 31-34% amino acid identity to the two types of retroviral duplicated protease sequences over a region encompassing 125 amino acid residues. Sequences flanking the vaccinia gene showed no significant homology at either the DNA or amino acid level to the retroviruses. Nuclease S1 and primer extension analyses determined that the vaccinia gene is transcribed early in infection.
逆转录病毒蛋白酶编码区（PR）位于gag基因和pol基因之间，在现今以1型猿猴逆转录病毒为代表的谱系中经历了基因复制；复制片段的序列与目前的PR序列有很大差异[鲍尔，医学博士，马克思，PA，布莱恩特，ML，加德纳，MB，巴尔，PJ和卢西夫，PA（1986年）《科学》231，1567 - 1572]。类似PR的复制基因片段在某个时候易位到慢病毒pol基因内的一个新位点（在1型人类免疫缺陷病毒分化之后），并在那里得以保留。我们在痘苗病毒基因组中鉴定出一个序列，它在一个开放阅读框中与两种逆转录病毒的类似PR的复制基因片段同源，其产物预计是一种16.2 kDa的蛋白质。痘苗病毒类似PR的基因位于HindIII F片段中，其产物在包含125个氨基酸残基的区域与两种逆转录病毒复制蛋白酶序列的氨基酸同一性为31 - 34%。痘苗病毒基因两侧的序列在DNA或氨基酸水平上与逆转录病毒均无明显同源性。核酸酶S1和引物延伸分析确定痘苗病毒基因在感染早期转录。
