Shchelkunov S N, Totmenin A V
Institute of Molecular Biology, Russian State Research Center NPO Vector, Koltsovo, Novosibirsk region.
Virus Genes. 1995 Feb;9(3):231-45. doi: 10.1007/BF01702879.
The genome nucleotide sequences of two strains of variola major virus and one strain of vaccinia virus were compared. One hundred and sixty-eight short (less than 100 bp in length) and eight long (more than 900 bp in length) deletions, four deletion/insertion regions, and four regions of multiple mutational differences between variola and vaccinia virus DNAs were revealed. Short deletions generally occur at directly repeated sequences of 3-21 bp. Long deletions showed no evidence of repeated sequences at their points of junction. We suggest the presence of a consensus sequence characteristic of these junctions and propose that there is a virus-encoded enzyme that produces this nonhomologous recombination/deletion in the cytoplasm of the infected cell.
对两株天花病毒和一株痘苗病毒的基因组核苷酸序列进行了比较。发现了168个短缺失（长度小于100bp）和8个长缺失（长度大于900bp）、4个缺失/插入区域以及天花病毒和痘苗病毒DNA之间4个多突变差异区域。短缺失通常发生在3 - 21bp的直接重复序列处。长缺失在其连接处没有重复序列的迹象。我们认为这些连接处存在一种共有序列特征，并提出在受感染细胞的细胞质中存在一种病毒编码的酶，该酶会产生这种非同源重组/缺失。
