Li Xin, Zhang Yuelin
Department of Botany, University of British Columbia, Vancouver, BC, Canada.
Department of Botany, University of British Columbia, Room #3529, 6270 University Boulevard, Vancouver, BC, Canada, V6T 1Z4.
Methods Mol Biol. 2016;1363:1-8. doi: 10.1007/978-1-4939-3115-6_1.
Genetic screens have proven to be a useful tool in the dissection of biological processes in plants. Specifically, suppressor screens have been widely used to study signal transduction pathways. Here we provide a detailed protocol for ethyl methanesulfonate (EMS) mutagenesis used in our suppressor screens in Arabidopsis and discuss the basic principles behind suppressor screen design and downstream analyses.
遗传筛选已被证明是剖析植物生物学过程的有用工具。具体而言，抑制子筛选已被广泛用于研究信号转导途径。在这里，我们提供了一份详细的方案，用于我们在拟南芥抑制子筛选中使用的甲磺酸乙酯（EMS）诱变，并讨论抑制子筛选设计和下游分析背后的基本原理。
