Baron Aleksandr, Lindsey Kimberly, Sidow Stephanie J, Dickinson Douglas, Chuang Augustine, McPherson James C
U.S. Army Dental Activity, Fort Gordon, Georgia.
U.S. Army Dental Activity, Fort Gordon, Georgia.
J Endod. 2016 Jan;42(1):145-9. doi: 10.1016/j.joen.2015.08.035. Epub 2015 Nov 11.
The purpose of this investigation was to determine the effect of a sodium hypochlorite-surfactant combination on the removal of Enterococcus faecalis from infected teeth.
Sixty-four extracted human single canal anterior teeth were prepared with rotary instrumentation and sterilized. Teeth were divided into 4 groups, N = 16. Three experimental groups were inoculated with E. faecalis and cultured for 21 days before use: positive control group, no irrigation; NaOCl group, irrigated with 5 mL 6% NaOCl; and NaOCl/BAK group, irrigated with 5 mL 6% NaOCl/0.008% benzalkonium chloride (BAK). The negative control group received medium only and no inoculate. Paper point sampling of the canals was obtained before irrigation (S1) for all 4 groups and for 2 groups after irrigation (S2) to determine remaining colony-forming units. After sampling, all teeth were split in half and evaluated for bacterial viability colony-forming units and penetration of dentinal tubules by using fluorescent vital dye staining and confocal laser scanning microscopy.
Comparison of pre-irrigation and post-irrigation paper point samples from the 2 irrigated groups showed a significant reduction in bacterial canal load (P < .001, Kruskal-Wallis), with a significantly lower load in the NaOCl/BAK group than in the NaOCl group (P = .001, Mann-Whitney U test); 68.8% of the NaOCl/BAK samples gave no recoverable counts. In contrast, no significant difference between these groups was found for counts recovered from dentin. Confocal laser scanning microscopy showed no differences in tubule penetration.
The addition of BAK to NaOCl significantly reduced the number of remaining bacteria within the canal after irrigation compared with NaOCl alone.
本研究的目的是确定次氯酸钠与表面活性剂组合对从感染牙齿中去除粪肠球菌的效果。
用旋转器械对64颗拔除的单根管人前牙进行预备并消毒。牙齿分为4组，每组16颗。3个实验组接种粪肠球菌并培养21天后使用：阳性对照组，不冲洗；次氯酸钠组，用5毫升6%次氯酸钠冲洗；次氯酸钠/苯扎氯铵组，用5毫升6%次氯酸钠/0.008%苯扎氯铵（BAK）冲洗。阴性对照组仅接受培养基且不接种。在冲洗前（S1）对所有4组以及冲洗后对2组进行根管纸尖取样，以确定剩余的菌落形成单位。取样后，将所有牙齿沿中线劈开，使用荧光活性染料染色和共聚焦激光扫描显微镜评估细菌活力菌落形成单位以及牙本质小管的渗透情况。
对2个冲洗组冲洗前后的纸尖样本进行比较，结果显示根管内细菌载量显著降低（P <.001，Kruskal-Wallis检验），次氯酸钠/苯扎氯铵组的载量显著低于次氯酸钠组（P =.001，Mann-Whitney U检验）；68.8%的次氯酸钠/苯扎氯铵样本未检测到可培养细菌。相比之下，从牙本质中回收的细菌数量在这些组之间没有显著差异。共聚焦激光扫描显微镜显示小管渗透情况无差异。
与单独使用次氯酸钠相比，在次氯酸钠中添加苯扎氯铵可显著减少冲洗后根管内残留细菌的数量。
