具有针对肺炎克雷伯菌裂解活性的新型T7样病毒vB_KpnP_KpV289的全基因组序列
Complete genome sequence of novel T7-like virus vB_KpnP_KpV289 with lytic activity against Klebsiella pneumoniae.
作者信息
Volozhantsev Nikolay V, Myakinina Vera P, Popova Anastasia V, Kislichkina Angelina A, Komisarova Ekaterina V, Knyazeva Anastasia I, Krasilnikova Valentina M, Fursova Nadezhda K, Svetoch Eduard A
机构信息
State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russian Federation.
出版信息
Arch Virol. 2016 Feb;161(2):499-501. doi: 10.1007/s00705-015-2680-z. Epub 2015 Nov 17.
A novel bacteriophage, vB_KpnP_KpV289, lytic for hypermucoviscous strains of Klebsiella pneumoniae, was attributed to the family Podoviridae, subfamily Autographivirinae, genus T7likevirus based on transmission electron microscopy and genome analysis. The complete genome of the bacteriophage vB_KpnP_KpV289 consists of a linear double-stranded DNA of 41,054 bp including 179-bp direct-repeat sequences at the ends and 51 open reading frames (ORFs). The G+C content is 52.56 %. The phage was shown to lyse 15 out of 140 (10.7 %) K. pneumoniae strains belonged to the capsular types K-1, K-2, and K-57 and strains without a determined capsular type, including a hypermucoviscous strain of the novel sequence type ST-1554.
一种新型噬菌体vB_KpnP_KpV289，可裂解肺炎克雷伯菌的高黏液性菌株，基于透射电子显微镜和基因组分析，它属于短尾噬菌体科、自剪接病毒亚科、T7样病毒属。噬菌体vB_KpnP_KpV289的完整基因组由一条41,054 bp的线性双链DNA组成，两端包含179 bp的直接重复序列和51个开放阅读框（ORF）。G+C含量为52.56%。该噬菌体可裂解140株肺炎克雷伯菌中的15株（10.7%），这些菌株属于荚膜型K-1、K-2和K-57以及未确定荚膜型的菌株，包括一株新型序列型ST-1554的高黏液性菌株。
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