Lim Jeong-A, Heu Sunggi, Park Jinwoo, Roh Eunjung
Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, 55365, Republic of Korea.
Research Group of Food Safety, Korea Food Research Institute, Sungnam, 13539, Republic of Korea.
Arch Virol. 2017 Aug;162(8):2441-2444. doi: 10.1007/s00705-017-3349-6. Epub 2017 Apr 13.
Bacteriophage vB_PcaP_PP2 (PP2) is a novel virulent phage that infects the plant-pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. PP2 phage has a 41,841-bp double-stranded DNA encoding 47 proteins, and it was identified as a member of the family Podoviridae by transmission electron microscopy. Nineteen of its open reading frames (ORFs) show homology to functional proteins, and 28 ORFs have been characterized as hypothetical proteins. PP2 phage is homologous to Cronobacter phage vB_CskP_GAP227 and Dev-CD-23823. Based on phylogenetic analysis, PP2 and its homologous bacteriophages form a new group within the subfamily Autographivirinae in the family Podoviridae, suggesting the need to establish a new genus. No lysogenic-cycle-related genes or bacterial toxins were identified.
噬菌体vB_PcaP_PP2（PP2）是一种新型烈性噬菌体，可感染植物致病细菌胡萝卜软腐果胶杆菌胡萝卜软腐亚种。PP2噬菌体具有一条41,841碱基对的双链DNA，编码47种蛋白质，通过透射电子显微镜鉴定为短尾噬菌体科的成员。其19个开放阅读框（ORF）与功能蛋白具有同源性，28个ORF被表征为假定蛋白。PP2噬菌体与阪崎肠杆菌噬菌体vB_CskP_GAP227和Dev-CD-23823具有同源性。基于系统发育分析，PP2及其同源噬菌体在短尾噬菌体科自剪接噬菌体亚科内形成一个新的组，这表明需要建立一个新属。未鉴定到与溶原周期相关的基因或细菌毒素。
