Brandl Holger, Moon HongKee, Vila-Farré Miquel, Liu Shang-Yun, Henry Ian, Rink Jochen C
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
Nucleic Acids Res. 2016 Jan 4;44(D1):D764-73. doi: 10.1093/nar/gkv1148. Epub 2015 Nov 17.
Planarian flatworms are in the midst of a renaissance as a model system for regeneration and stem cells. Besides two well-studied model species, hundreds of species exist worldwide that present a fascinating diversity of regenerative abilities, tissue turnover rates, reproductive strategies and other life history traits. PlanMine (http://planmine.mpi-cbg.de/) aims to accomplish two primary missions: First, to provide an easily accessible platform for sharing, comparing and value-added mining of planarian sequence data. Second, to catalyze the comparative analysis of the phenotypic diversity amongst planarian species. Currently, PlanMine houses transcriptomes independently assembled by our lab and community contributors. Detailed assembly/annotation statistics, a custom-developed BLAST viewer and easy export options enable comparisons at the contig and assembly level. Consistent annotation of all transcriptomes by an automated pipeline, the integration of published gene expression information and inter-relational query tools provide opportunities for mining planarian gene sequences and functions. For inter-species comparisons, we include transcriptomes of, so far, six planarian species, along with images, expert-curated information on their biology and pre-calculated cross-species sequence homologies. PlanMine is based on the popular InterMine system in order to make the rich biology of planarians accessible to the general life sciences research community.
涡虫作为再生和干细胞的模型系统正处于复兴之中。除了两个经过充分研究的模式物种外,全球还存在数百个物种,它们展现出了再生能力、组织更新率、繁殖策略和其他生活史特征的迷人多样性。PlanMine(http://planmine.mpi-cbg.de/)旨在完成两项主要任务:第一,提供一个易于访问的平台,用于涡虫序列数据的共享、比较和增值挖掘。第二,促进涡虫物种间表型多样性的比较分析。目前,PlanMine收录了由我们实验室和社区贡献者独立组装的转录组。详细的组装/注释统计、定制开发的BLAST查看器和便捷的导出选项,使得在重叠群和组装水平上进行比较成为可能。通过自动化流程对所有转录组进行一致注释、整合已发表的基因表达信息以及相互关联的查询工具,为挖掘涡虫基因序列和功能提供了机会。对于种间比较,我们收录了目前六个涡虫物种的转录组,以及图像、关于它们生物学的专家整理信息和预先计算的跨物种序列同源性。PlanMine基于广受欢迎的InterMine系统,以便让普通生命科学研究群体能够了解涡虫丰富的生物学特性。