Department of Bacteriology, University of Wisconsin - Madison, 1531 Microbial Sciences Building, 1550 Linden Drive, Madison, WI 53706, United States; Great Lakes Bioenergy Research Center, University of Wisconsin - Madison, Madison, WI 53726, United States.
Department of Bacteriology, University of Wisconsin - Madison, 1531 Microbial Sciences Building, 1550 Linden Drive, Madison, WI 53706, United States; Great Lakes Bioenergy Research Center, University of Wisconsin - Madison, Madison, WI 53726, United States; Institute for Microbial and Biochemical Technology, Forest Products Laboratory, USDA Forest Service, Madison, WI 53726, United States.
Bioresour Technol. 2016 Jan;200:780-8. doi: 10.1016/j.biortech.2015.10.104. Epub 2015 Nov 4.
Lipid production by oleaginous yeasts is optimal at high carbon-to-nitrogen ratios. In the current study, nitrogen and carbon consumption by Lipomyces starkeyi were directly measured in defined minimal media with nitrogen content and agitation rates as variables. Shake flask cultures with an initial C:N ratio of 72:1 cultivated at 200rpm resulted in a lipid output of 10g/L, content of 55%, yield of 0.170g/g, and productivity of 0.06g/L/h. All of these values decreased by ≈50-60% when the agitation rate was raised to 300rpm or when the C:N ratio was lowered to 24:1, demonstrating the importance of these parameters. Under all conditions, L. starkeyi cultures tolerated acidified media (pH≈2.6) without difficulty, and produced considerable amounts of alcohols; including ethanol, mannitol, arabitol, and 2,3-butanediol. L. starkeyi also produced lipids from a corn stover hydrolysate, showing its potential to produce biofuels from renewable agricultural feedstocks.
产油酵母的油脂生产在高碳氮比下最佳。在本研究中，通过直接测量氮含量和搅拌速度作为变量的定义最小培养基中的氮和碳消耗，来测量拉斯克酵母的氮和碳消耗。在初始 C:N 比为 72:1 的摇瓶培养物中，以 200rpm 培养，得到 10g/L 的油脂产量、55%的含量、0.170g/g 的产率和 0.06g/L/h 的生产力。当搅拌速度提高到 300rpm 或 C:N 比降低到 24:1 时，所有这些值都会降低约 50-60%，这表明这些参数很重要。在所有条件下，拉斯克酵母培养物都能轻松耐受酸化介质（pH≈2.6），并产生大量醇；包括乙醇、甘露醇、赤藻糖醇和 2,3-丁二醇。拉斯克酵母还可以从玉米秸秆水解物中生产油脂，显示出其从可再生农业原料生产生物燃料的潜力。
