Sapsirisuk Sirawich, Polburee Pirapan, Lorliam Wanlapa, Limtong Savitree
Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
J Fungi (Basel). 2022 Oct 18;8(10):1100. doi: 10.3390/jof8101100.
As an interesting alternative microbial platform for the sustainable synthesis of oleochemical building blocks and biofuels, oleaginous yeasts are increasing in both quantity and diversity. In this study, oleaginous yeast species from northern Thailand were discovered to add to the topology. A total of 127 yeast strains were isolated from 22 forest soil samples collected from mountainous areas. They were identified by an analysis of the D1/D2 domain of the large subunit rRNA (LSU rRNA) gene sequences to be 13 species. The most frequently isolated species were and . Based on the cellular lipid content determination, 78 strains of ten yeast species, and two potential new yeast that which accumulated over 20% of dry biomass, were found to be oleaginous yeast strains. Among the oleaginous species detected, and have never been reported as oleaginous yeast before. In addition, none of the species in the genera and were found to be oleaginous yeast. SWU-NGP 2-5 accumulated the highest lipid content of 74.26% dry biomass, whereas SWU-NGP 14-6 revealed the highest lipid quantity at 5.20 ± 0.03 g L. The fatty acid profiles of the selected oleaginous yeasts varied depending on the strain and suitability for biodiesel production.
作为一种用于可持续合成油脂化学基础原料和生物燃料的有趣的替代性微生物平台，产油酵母在数量和多样性上都在增加。在本研究中，发现来自泰国北部的产油酵母物种增加了这种拓扑结构。从山区采集的22份森林土壤样品中总共分离出127株酵母菌株。通过对大亚基rRNA（LSU rRNA）基因序列的D1/D2结构域进行分析，确定它们为13个物种。最常分离出的物种是 和 。基于细胞脂质含量测定，发现属于10个酵母物种的78株菌株以及两种积累超过20%干生物量的潜在新酵母为产油酵母菌株。在所检测到的产油物种中， 和 以前从未被报道为产油酵母。此外，在 属和 属中没有发现任何物种是产油酵母。SWU-NGP 2-5积累的脂质含量最高，为干生物量的74.26%，而SWU-NGP 14-6的脂质产量最高，为5.20±0.03 g/L。所选产油酵母的脂肪酸谱因菌株而异，且对生物柴油生产的适用性也不同。
