Go Young-Mi, Liang Yongliang, Uppal Karan, Soltow Quinlyn A, Promislow Daniel E L, Wachtman Lynn M, Jones Dean P
Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Clinical Biomarkers Laboratory, Emory University, Atlanta, Georgia, 30322, United States of America.
Department of Pathology and Department of Biology, University of Washington, Seattle, Washington, 98195, United States of America.
PLoS One. 2015 Nov 18;10(11):e0142916. doi: 10.1371/journal.pone.0142916. eCollection 2015.
High-resolution metabolomics has created opportunity to integrate nutrition and metabolism into genetic studies to improve understanding of the diverse radiation of primate species. At present, however, there is very little information to help guide experimental design for study of wild populations. In a previous non-targeted metabolomics study of common marmosets (Callithrix jacchus), Rhesus macaques, humans, and four non-primate mammalian species, we found that essential amino acids (AA) and other central metabolites had interspecies variation similar to intraspecies variation while non-essential AA, environmental chemicals and catabolic waste products had greater interspecies variation. The present study was designed to test whether 55 plasma metabolites, including both nutritionally essential and non-essential metabolites and catabolic products, differ in concentration in common marmosets and humans. Significant differences were present for more than half of the metabolites analyzed and included AA, vitamins and central lipid metabolites, as well as for catabolic products of AA, nucleotides, energy metabolism and heme. Three environmental chemicals were present at low nanomolar concentrations but did not differ between species. Sex and age differences in marmosets were present for AA and nucleotide metabolism and warrant additional study. Overall, the results suggest that quantitative, targeted metabolomics can provide a useful complement to non-targeted metabolomics for studies of diet and environment interactions in primate evolution.
高分辨率代谢组学为将营养与代谢整合到基因研究中创造了机会，以增进对灵长类物种多样辐射的理解。然而，目前几乎没有信息可用于指导野生种群研究的实验设计。在之前一项对普通狨猴（Callithrix jacchus）、恒河猴、人类以及四种非灵长类哺乳动物的非靶向代谢组学研究中，我们发现必需氨基酸（AA）和其他中心代谢物的种间差异与种内差异相似，而非必需氨基酸、环境化学物质和分解代谢废物的种间差异更大。本研究旨在测试55种血浆代谢物（包括营养必需和非必需代谢物以及分解代谢产物）在普通狨猴和人类中的浓度是否存在差异。分析的代谢物中超过一半存在显著差异，包括氨基酸、维生素和中心脂质代谢物，以及氨基酸、核苷酸、能量代谢和血红素的分解代谢产物。三种环境化学物质以低纳摩尔浓度存在，但物种间无差异。狨猴在氨基酸和核苷酸代谢方面存在性别和年龄差异，值得进一步研究。总体而言，结果表明，定量靶向代谢组学可为灵长类动物进化中饮食与环境相互作用的研究提供非靶向代谢组学的有益补充。
