Short Michael A, Wang Wenbo, Tai Isabella T, Zeng Haishan
Imaging Unit - Integrative Oncology Department, BC Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, B.C., Canada, V5Z 1L3.
Division of Gastroenterology, University of British Columbia and Genome Sciences Centre, BC Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, B.C., Canada, V5Z 1L3.
J Biophotonics. 2016 Jan;9(1-2):44-8. doi: 10.1002/jbio.201500205. Epub 2015 Nov 20.
The objective of this study was to build and test an adjunct system to a colonoscope for in vivo measurement of Raman spectra from colon tissue for potentially improving the detection of early cancers. The novelty of this system was that low cost fibre optic probes were used, without the addition of expensive optical filters. Good quality in vivo Raman spectra were successfully obtained with a 1 s integration time in the high frequency (HF) range from normal tissue and polyps of patients during a colonoscopy. The polyps were subsequently removed, and their pathology determined. The acquired in vivo Raman spectra showed clear changes between tissue with normal and tubular adenoma pathology. Further clinical study with this low cost HF Raman probe is warranted to fully test its clinical utility.
本研究的目的是构建并测试一种用于结肠镜的辅助系统，以在体内测量结肠组织的拉曼光谱，从而有可能改善早期癌症的检测。该系统的新颖之处在于使用了低成本的光纤探头，且未添加昂贵的光学滤光片。在结肠镜检查期间，以1秒的积分时间成功获得了患者正常组织和息肉高频（HF）范围内的高质量体内拉曼光谱。随后切除息肉并确定其病理情况。所采集的体内拉曼光谱显示，具有正常病理和管状腺瘤病理的组织之间存在明显变化。有必要使用这种低成本的高频拉曼探头进行进一步的临床研究，以全面测试其临床效用。
