Horgan Conor C, Jensen Magnus, Chiappini Ciro, Vercauteren Tom, Cook Richard, Bergholt Mads S
Centre for Craniofacial and Regenerative Biology, King's College London, London SE1 9RT, UK.
London Centre for Nanotechnology, King's College London, London WC2R 2LS, UK.
Biomed Opt Express. 2022 Mar 21;13(4):2278-2285. doi: 10.1364/BOE.449110. eCollection 2022 Apr 1.
Confocal laser endomicroscopy (CLE) offers imaging of tissue microarchitecture and has emerged as a promising tool for clinical diagnosis of cancer across many organs. CLE, however, can show high inter-observer dependency and does not provide information about tissue molecular composition. In contrast, Raman spectroscopy is a label-free optical technique that provides detailed biomolecular compositional information but offers limited or no morphological information. Here we present a novel hybrid fiber-optic confocal Raman endomicroscopy system for morpho-chemical tissue imaging and analysis. The developed confocal endomicroscopy system is based on a novel detection scheme for rejecting Raman silica fiber interference permitting simultaneous CLE imaging and Raman spectral acquisition of tissues through a coherent fiber bundle. We show that this technique enables real-time microscopic visualization of tissue architecture as well as simultaneous pointwise label-free biomolecular characterization and fingerprinting of tissue paving the way for multimodal diagnostics at endoscopy.
共聚焦激光内镜显微镜（CLE）可对组织微观结构进行成像，已成为跨多个器官进行癌症临床诊断的一种有前景的工具。然而，CLE可能显示出较高的观察者间依赖性，并且无法提供有关组织分子组成的信息。相比之下，拉曼光谱是一种无标记光学技术，可提供详细的生物分子组成信息，但形态学信息有限或无法提供。在此，我们展示了一种用于形态化学组织成像和分析的新型混合光纤共聚焦拉曼内镜显微镜系统。所开发的共聚焦内镜显微镜系统基于一种新型检测方案，用于排除拉曼石英光纤干扰，从而能够通过相干光纤束同时对组织进行CLE成像和拉曼光谱采集。我们表明，该技术能够实时微观可视化组织结构，同时对组织进行逐点无标记生物分子表征和指纹识别，为内镜检查中的多模态诊断铺平了道路。
