Liu Yang, Zhao Ran, Wang Ronghuan, Yao Su, Zhai Lei, Zhang Xin, Chen Chuanyong, Cao Yanhua, Xu Tianjun, Ge Yuanyuan, Zhao Jiuran, Cheng Chi
Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, People's Republic of China.
China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing, 100015, People's Republic of China.
Antonie Van Leeuwenhoek. 2016 Feb;109(2):207-13. doi: 10.1007/s10482-015-0622-0. Epub 2015 Nov 20.
Four Gram-stain positive bacterial strains, designated as 4R1(T), 4R9, 4L13 and 4L18, isolated from seeds of hybrid maize (Zea mays L., Jingke 968), were investigated using a polyphasic taxonomic approach. The cells were found to be facultatively aerobic, motile, spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates should be recognised as a species of the genus Paenibacillus, with two close neighbours being Paenibacillus nicotianae YIM h-19(T) (98.41 % similarity) and Paenibacillus hordei RH-N24(T) (98.37 %). The DNA G+C content of strain 4R1(T) was determined to be 51.6 mol %. Its polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid. The predominant respiratory quinone was identified as MK-7 and the major fatty acids were found to be anteiso-C15:0, anteiso-C12:0, anteiso-C13:0 and anteiso-C11:0. Strains 4R1(T), 4R9, 4L13 and 4L18 were clearly distinguished from the reference type strains using phylogenetic analysis, DNA-DNA hybridization and a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strains 4R1(T), 4R9, 4L13 and 4L18 represent a novel species of the genus Paenibacillus, for which the name Paenibacillus chinensis sp. nov. is proposed. The type strain is 4R1(T) (=KCTC 33672(T) = CICC 23864(T)).
从杂交玉米（玉米，京科968）种子中分离出4株革兰氏染色阳性细菌菌株，分别命名为4R1(T)、4R9、4L13和4L18，采用多相分类方法对其进行了研究。发现这些细胞为兼性需氧、运动、产芽孢且呈杆状。基于16S rRNA基因序列的系统发育分析表明，这些分离株应被鉴定为芽孢杆菌属的一个种，其两个近缘种为烟草芽孢杆菌YIM h-19(T)（相似度98.41%）和大麦芽孢杆菌RH-N24(T)（相似度98.37%）。菌株4R1(T)的DNA G+C含量测定为51.6 mol%。发现其极性脂质谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的脂质组成。主要呼吸醌被鉴定为MK-7，主要脂肪酸为anteiso-C15:0、anteiso-C12:0、anteiso-C13:0和anteiso-C11:0。通过系统发育分析、DNA-DNA杂交以及一系列生理生化特征，菌株4R1(T)、4R9、4L13和4L18与参考模式菌株明显区分开来。从基因型和表型数据可以明显看出，菌株4R1(T)、4R9、4L13和4L18代表芽孢杆菌属的一个新种，为此提出新种名为中华芽孢杆菌（Paenibacillus chinensis sp. nov.）。模式菌株为4R1(T)（=KCTC 33672(T) = CICC 23864(T)）。
