Liu Yang, Li Nannan, Eom Mi Kyung, Schumann Peter, Zhang Xin, Cao Yanhua, Ge Yuanyuan, Xiao Ming, Zhao Jiuran, Cheng Chi, Kim Song-Gun
China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China.
Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, PR China.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4606-4611. doi: 10.1099/ijsem.0.002341. Epub 2017 Oct 6.
Two Gram-stain-positive bacterial strains, designated as 5L6 and 6L6, isolated from seeds of hybrid maize (Zea mays L., Jingke 968) were investigated using a polyphasic taxonomic approach. The cells were aerobic, motile, endospore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were recognized as a species of the genus Bacillus, to which the five closest neighbours are Bacillus solani FJAT-18043 (99.8 % similarity), Bacillus horneckiae DSM 23495 (97.7 %), Bacillus eiseniae A1-2 (97.4 %), Bacillus kochii WCC 4582 (97.1 %) and Bacillus purgationiresistens DS22 (97.0 %). The DNA G+C content of strain 5L6 was 37.4 mol%. Its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was MK-7 and the major fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C14 : 0, anteiso-C17 : 0 and C16 : 1 ω7c alcohol. The cell-wall peptidoglycan contained ornithine, serine, aspartic acid, glutamic acid and alanine while diaminopimelic acid could not be detected. Strains 5L6 and 6L6 were clearly distinguished from the type strains of related validly named species using phylogenetic analysis, DNA-DNA hybridization, fatty acid analysis, peptidoglycan analysis and comparison of a range of physiological and biochemical characteristics. The genotypic and phenotypic data show that strains 5L6 and 6L6 represent a novel species of the genus Bacillus, for which the name Bacillusciccensis sp. nov. is proposed. The type strain is 5L6 (=KCTC 33663=CICC 23855=DSM 104513).
采用多相分类方法对从杂交玉米（Zea mays L.，京科968）种子中分离得到的两株革兰氏阳性细菌菌株5L6和6L6进行了研究。这些细胞为需氧、运动、产芽孢且呈杆状。基于16S rRNA基因序列的系统发育分析表明，这些分离株被鉴定为芽孢杆菌属的一个种，与其亲缘关系最近的五个种分别是茄芽孢杆菌FJAT-18043（相似度99.8%）、霍氏芽孢杆菌DSM 23495（97.7%）、艾氏芽孢杆菌A1-2（97.4%）、科氏芽孢杆菌WCC 4582（97.1%）和抗净化芽孢杆菌DS22（97.0%）。菌株5L6的DNA G+C含量为37.4 mol%。其极性脂谱由双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺组成。主要呼吸醌为MK-7，主要脂肪酸为异-C15:0、anteiso-C15:0、异-C16:0、异-C14:0、anteiso-C17:0和C16:1 ω7c醇。细胞壁肽聚糖含有鸟氨酸、丝氨酸、天冬氨酸、谷氨酸和丙氨酸，未检测到二氨基庚二酸。通过系统发育分析、DNA-DNA杂交、脂肪酸分析、肽聚糖分析以及一系列生理生化特征的比较，菌株5L6和6L6与相关有效命名种的模式菌株明显区分开来。基因型和表型数据表明，菌株5L6和6L6代表芽孢杆菌属的一个新种，为此提出新名称环芽孢杆菌（Bacillus ciccensis sp. nov.）。模式菌株为5L6（=KCTC 33663=CICC 23855=DSM 104513）。
