Liu Yang, Zhai Lei, Wang Ronghuan, Zhao Ran, Zhang Xin, Chen Chuanyong, Cao Yu, Cao Yanhua, Xu Tianjun, Ge Yuanyuan, Zhao Jiuran, Cheng Chi
China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing 100015, PR China.
Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, PR China.
Int J Syst Evol Microbiol. 2015 Dec;65(12):4533-4538. doi: 10.1099/ijsem.0.000608. Epub 2015 Sep 14.
Four Gram-stain-positive bacterial strains, designated 6R2T, 6R18, 3T2 and 3T10, isolated from seeds of hybrid maize (Zea mays L., Jingke 968) were investigated using a polyphasic taxonomic approach. Cells were aerobic, motile, spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolates may represent a novel species of the genus Paenibacillus, the four closest neighbours being Paenibacillus lautus NRRL NRS-666T (97.1 % similarity), Paenibacillus glucanolyticus DSM 5162T (97.0 %), Paenibacillus lactis MB 1871T (97.0 %) and Paenibacillus chibensis JCM 9905T (96.8 %). The DNA G+C content of strain 6R2T was 51.8 mol%. Its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C15 : 0 and iso-C14 : 0. Strains 6R2T, 6R18, 3T2 and 3T10 were clearly distinguished from the above type strains using phylogenetic analysis, DNA-DNA hybridization, and a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strains 6R2T, 6R18, 3T2 and 3T10 represent a novel species of the genus Paenibacillus, for which the name Paenibacillus zeae sp. nov. is proposed. The type strain is 6R2T ( = KCTC 33674T = CICC 23860T).
采用多相分类方法对从杂交玉米（玉米，京科968）种子中分离出的4株革兰氏阳性细菌菌株（分别命名为6R2T、6R18、3T2和3T10）进行了研究。细胞为需氧、运动、产芽孢且呈杆状。基于16S rRNA基因序列的系统发育分析表明，这些分离株可能代表芽孢杆菌属的一个新物种，其四个最相近的亲缘种为迟缓芽孢杆菌NRRL NRS - 666T（相似度97.1%）、解葡聚糖芽孢杆菌DSM 5162T（97.0%）、乳酸芽孢杆菌MB 1871T（97.0%）和济州芽孢杆菌JCM 9905T（96.8%）。菌株6R2T的DNA G + C含量为51.8 mol%。其极性脂谱由双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺组成。主要呼吸醌为甲基萘醌7（MK - 7），主要脂肪酸为anteiso - C15∶0和iso - C14∶0。通过系统发育分析、DNA - DNA杂交以及一系列生理生化特征，菌株6R2T、6R18、3T2和3T10与上述模式菌株明显区分开来。从基因型和表型数据可以明显看出，菌株6R2T、6R18、3T2和3T10代表芽孢杆菌属的一个新物种，为此提出新名称玉米芽孢杆菌（Paenibacillus zeae sp. nov.）。模式菌株为6R2T（= KCTC 33674T = CICC 23860T）。
