尖叶琼楠、香椒、光叶新木姜子和密脉鹅掌柴甲醇提取物对多重耐药革兰氏阴性菌的体外抗菌及抗生素增效活性
In vitro antibacterial and antibiotic-potentiation activities of the methanol extracts from Beilschmiedia acuta, Clausena anisata, Newbouldia laevis and Polyscias fulva against multidrug-resistant Gram-negative bacteria.
作者信息
Tankeo Simplice B, Tane Pierre, Kuete Victor
机构信息
Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Laboratory of Natural Products Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
出版信息
BMC Complement Altern Med. 2015 Nov 22;15:412. doi: 10.1186/s12906-015-0944-5.
BACKGROUND
The present study was designed to investigate the antibacterial activities of the methanol extracts from different parts of Beilschmedia acuta Kosterm (Lauraceae), Clausena anisata (Willd) Hook (Rutaceae), Newbouldia laevis Seem (Bignoniaceae) and Polyscias fulva (Hiern) Harms (Araliaceae) as well as their synergistic effects with antibiotics against a panel of Gram-negative bacteria, including multi-drug resistant (MDR) phenotypes expressing active efflux pumps.
METHODS
Broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the extracts, as well as those of antibiotics in association with the most active ones, B. acuta, N. laevis and P. fulva.
RESULTS
MIC values obtained indicate that extracts from the bark of B. acuta were active on all the 26 tested Gram-negative bacteria, with MICs ranging from values below 8 to 256 μg/mL. Other samples displayed selective activities, their inhibitory effects being observed on 9 (34.62 %) of the 26 bacterial strains for N. laevis leaves extract, 6 (23.10 %) for both C. anisata leaves and roots extracts, 7 (26.9 %) and 4 (15.4 %) for leaves and roots extracts of P. fulva respectively. Extract from B. actua bark displayed the best antibacterial activity with MIC values below 100 μg/mL against 16 (61.5 %) of the 26 tested microorganisms. The lowest MIC values (below 8 μg/mL) were obtained with this extract against Escherichia coli W3110 and Klebsiella pneumoniae ATCC11296. The MIC values of this extract were lower than those of ciprofloxacin against E. coli W3110, Enterobacter aerogenes ATCC13048, CM64 and Providencia stuartii NAE16. At MIC/2, the best percentages of synergistic effects (100 %), were obtained with B. acuta bark extract and tetracycline (TET) as well as with P. fulva leaves extract and TET and kanamycin (KAN).
CONCLUSION
The overall results of the present study provide information for the possible use of the studied plants and mostly Beilschmedia acuta in the control of bacterial infections including MDR phenotypes.
背景
本研究旨在调查尖叶琼楠（樟科）、香肉豆蔻（芸香科）、光叶纽敦豆（紫葳科）和密脉鹅掌柴（五加科）不同部位甲醇提取物的抗菌活性，以及它们与抗生素对一组革兰氏阴性菌的协同作用，包括表达活性外排泵的多重耐药（MDR）表型。
方法
采用肉汤微量稀释法测定提取物以及与活性最强的尖叶琼楠、光叶纽敦豆和密脉鹅掌柴联合使用的抗生素的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。
结果
所得MIC值表明，尖叶琼楠树皮提取物对所有26株受试革兰氏阴性菌均有活性，MIC范围为8 μg/mL以下至256 μg/mL。其他样品表现出选择性活性，光叶纽敦豆叶提取物对26株细菌中的9株（34.62%）有抑制作用，香肉豆蔻叶和根提取物对6株（23.10%）有抑制作用，密脉鹅掌柴叶和根提取物分别对7株（26.9%）和4株（15.4%）有抑制作用。尖叶琼楠树皮提取物表现出最佳抗菌活性，对26株受试微生物中的16株（61.5%）的MIC值低于100 μg/mL。该提取物对大肠杆菌W3110和肺炎克雷伯菌ATCC11296的MIC值最低（低于8 μg/mL）。该提取物对大肠杆菌W3110、产气肠杆菌ATCC13048、CM64和斯氏普罗威登斯菌NAE16的MIC值低于环丙沙星。在MIC/2时，尖叶琼楠树皮提取物与四环素（TET）以及密脉鹅掌柴叶提取物与TET和卡那霉素（KAN）的协同效应百分比最高（100%）。
结论
本研究的总体结果为所研究植物尤其是尖叶琼楠在控制包括MDR表型在内的细菌感染方面的可能应用提供了信息。
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