Department of Biochemistry, Faculty of science, University of Dschang, Cameroon.
BMC Complement Altern Med. 2011 Nov 1;11:104. doi: 10.1186/1472-6882-11-104.
The emergence of multi-drug resistant (MDR) phenotypes is a major public health problem today in the treatment of bacterial infections. The present study was designed to evaluate the antibacterial activities of the methanol extracts of eleven Cameroonian spices on a panel of twenty nine Gram negative bacteria including MDR strains.
The phytochemical analysis of the extracts was carried out by standard tests meanwhile the liquid micro-broth dilution was used for all antimicrobial assays.
Phytochemical analysis showed the presence of alkaloids, phenols and tannins in all plants extracts. The results of the antibacterial assays indicated that all tested extracts exert antibacterial activities, with the minimum inhibitory concentration (MIC) values varying from 32 to 1024 μg/ml. The extracts from Dichrostachys glomerata, Beilschmiedia cinnamomea, Aframomum citratum, Piper capense, Echinops giganteus, Fagara xanthoxyloïdes and Olax subscorpioïdea were the most active. In the presence of efflux pump inhibitor, PAßN, the activity of the extract from D. glomerata significantly increased on 69.2% of the tested MDR bacteria. At MIC/5, synergistic effects were noted with the extract of D. glomerata on 75% of the tested bacteria for chloramphenicol (CHL), tetracycline (TET) and norfloxacin (NOR). With B. cinnamomea synergy were observed on 62.5% of the studied MDR bacteria with CHL, cefepime (FEP), NOR and ciprofloxacin (CIP) and 75% with erythromycin (ERY).
The overall results provide information for the possible use of the studied extracts of the spices in the control of bacterial infections involving MDR phenotypes.
多药耐药（MDR）表型的出现是当今治疗细菌感染的一个主要公共卫生问题。本研究旨在评估 11 种喀麦隆香料的甲醇提取物对包括 MDR 菌株在内的 29 种革兰氏阴性菌的抗菌活性。
采用标准试验对提取物进行植物化学分析，同时采用液体微量肉汤稀释法进行所有抗菌测定。
植物化学分析表明，所有植物提取物均含有生物碱、酚类和单宁。抗菌测定结果表明，所有测试提取物均具有抗菌活性，最小抑菌浓度（MIC）值范围为 32 至 1024 μg/ml。来自双色钟萼木、肉桂、香附、山菅兰、刺山柑、黄华柳和臭常山的提取物最为活跃。在流出泵抑制剂 PAßN 的存在下，D. glomerata 提取物对 69.2%的测试 MDR 细菌的活性显著增加。在 MIC/5 时，与 D. glomerata 提取物观察到协同作用，对 75%的测试细菌的氯霉素（CHL）、四环素（TET）和诺氟沙星（NOR）有效。与肉桂提取物有协同作用的有 62.5%的研究 MDR 细菌对 CHL、头孢吡肟（FEP）、NOR 和环丙沙星（CIP），对红霉素（ERY）有 75%的协同作用。
总体结果为控制涉及 MDR 表型的细菌感染可能使用研究的香料提取物提供了信息。
