Byzova N A, Zherdev A V, Sveshnikov P G, Sadykhov E G, Dzantiev B B
Prikl Biokhim Mikrobiol. 2015 Sep-Oct;51(5):520-30.
A test system based on immunochromatography in the sandwich format and intended for express detection of Helicobacter pylori antigens has been developed. Contact of a sample with a test strip coated with immunochemical reagents triggers the movement of the liquid along the membrane components of the test strip, immunochemical interactions, and the formation of detection zones stained by gold nanoparticles. The concentration and kinetic dependences of the immunochemical interactions have been characterized. The reagent and membrane composition of the test system has been selected to provide a minimal detection limit. The detection of H. pylori cell wall antigens at concentrations as low as 0.3 μg/mL in aqueous solution and a suspension of a clinical sample of feces has been demonstrated; the assay duration was 10 minutes. Staining enhancement by the addition of silver salts allowed for a further reduction of the detection limit to 0.03 μg/mL. The developed test system can be used for field diagnostics.
已开发出一种基于夹心式免疫层析的检测系统，用于快速检测幽门螺杆菌抗原。样品与涂有免疫化学试剂的测试条接触会触发液体沿测试条的膜组件移动、免疫化学反应，并形成由金纳米颗粒染色的检测区。已对免疫化学反应的浓度和动力学依赖性进行了表征。选择了测试系统的试剂和膜组成以提供最低检测限。已证明在水溶液和粪便临床样品悬浮液中可检测到低至0.3μg/mL浓度的幽门螺杆菌细胞壁抗原；检测时间为10分钟。添加银盐增强染色可将检测限进一步降低至0.03μg/mL。所开发的测试系统可用于现场诊断。
