Werawatganon Duangporn, Pongsuchart Mongkol, Sereemaspun Amornpun, Hanvivatvong Orrawadee, Siriviriyakul Prasong
J Med Assoc Thai. 2015 Jan;98 Suppl 1:S85-90.
Helicobacter pylori (H. pylori) plays an important role in the development of chronic gastritis, peptic ulcer diseases, gastric adenocarcinoma, and gastric mucosal associated lymphoid tissue (MALT) lymphoma. The standard methods of bacterial staining, bacterial culture, and urease testfor the detection of H. pylori are time consuming and invasive. Non-invasive testing plays a significant role in the test-and-treat approach to H. pylori management. Lateral flow immunoassay (LFIA) is a promising method for pathogenic detection that is fast, easy to use, and low cost. In the present study, the authors developed an H. pylori LFIA strip using gold nanoparticles for H. pylori detection. The results reported that 20 μg of anti-H. pylori antibody mixed with 1 mL AuNPs solution and incubated for 2 hours was the best concentration preparation for application coverage over the gold nanoparticle surface. The limit of detection observable by the naked eye was 15 μg of H. pylori protein lysate. The findings of this study suggest the possible future development of an H. pylori LFLA strip for fast, easy to use, and low-cost diagnostic testing.
幽门螺杆菌（H. pylori）在慢性胃炎、消化性溃疡疾病、胃腺癌以及胃黏膜相关淋巴组织（MALT）淋巴瘤的发展过程中起着重要作用。用于检测幽门螺杆菌的细菌染色、细菌培养和尿素酶试验等标准方法既耗时又具有侵入性。非侵入性检测在幽门螺杆菌管理的检测与治疗方法中发挥着重要作用。侧向流动免疫分析（LFIA）是一种很有前景的病原体检测方法，具有快速、易用且成本低的特点。在本研究中，作者开发了一种使用金纳米颗粒检测幽门螺杆菌的LFIA试纸条。结果报告称，将20μg抗幽门螺杆菌抗体与1mL金纳米颗粒溶液混合并孵育2小时，是在金纳米颗粒表面实现应用覆盖的最佳浓度制备方法。肉眼可观察到的检测限为15μg幽门螺杆菌蛋白裂解物。本研究结果表明，未来可能开发出一种用于快速、易用且低成本诊断检测的幽门螺杆菌LFIA试纸条。
