Smirnova D N, Bogacheva N V, Darmov I V
The Federal State Budget Educational Institution of Higher Education "The Vyatka State University", 610000, Vyatka, Russia.
Klin Lab Diagn. 2018;63(4):242-246. doi: 10.18821/0869-2084-2018-63-4-242-246.
The immunochromatographic test-system was developed for detecting protein of pathogenicity of CagA Helicobacter pylori in various biological samples (feces, content of dento-gingival recesses) and also in culture of H.pylori. The test-system represents multi-membrane composite on the basis of membranes manufactured by "MDI" (India). The main immunochemical components of test-system are conjugate of nanoparticles of colloid gold with size of 30 nm with monoclonal antibodies (clone HP-387), applied to membrane for conjugate; monoclonal antibodies (clone HP-1811) and anti-species antibodies of goat against Ig of mouse, applied to nitrocellulose membrane correspondingly in test and control zones. All antibodies are produced by the firm "Bialeksa" (Russia).
免疫层析检测系统是为检测各种生物样本（粪便、龈沟内容物）以及幽门螺杆菌培养物中幽门螺杆菌CagA致病性蛋白而研发的。该检测系统是基于“MDI”（印度）生产的膜制成的多膜复合材料。检测系统的主要免疫化学成分为：粒径30nm的胶体金纳米颗粒与单克隆抗体（克隆号HP - 387）的结合物，应用于结合物膜；单克隆抗体（克隆号HP - 1811）以及山羊抗小鼠Ig抗种属抗体，分别应用于硝酸纤维素膜的检测区和对照区。所有抗体均由“Bialeksa”公司（俄罗斯）生产。
