Liu Xiuyun, Lin Jia, Zhang Yinglan, Peng Xiaolan, Guo Ning, Li Qiang
Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
Center for Chinese Medical Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.
Neurotoxicol Teratol. 2016 Jan-Feb;53:41-7. doi: 10.1016/j.ntt.2015.11.008. Epub 2015 Nov 18.
Antiepileptic effects of diphenylhydantoin (DPH) have been documented in animal studies and clinical research, while little is known about the effects of the drug on basic behaviors and anxiety-related behaviors. In order to understand neuroactivities of DPH deeply and administrate DPH in clinic rationally, it is necessary to study neurobehavioral effects of the drug. In the present study, the effects of DPH on the locomotor activity and thigmotaxis of zebrafish larvae at 5 days post fertilization (dpf) were explored under different illumination conditions. The influence of DPH on zebrafish larval responses to visual stimuli (sudden illumination transition from light to dark) was also investigated. Under light or dark condition, exposure to high concentrations of DPH resulted in decreased locomotor activity and thigmotaxis, whereas DPH treatment at low doses enhanced the locomotor activity. Additionally, sudden illumination transition induced robust increase in the locomotor activity and this phenomenon was not modified by DPH treatment. Our results suggest that DPH has potential stimulatory and inhibitory effects on the locomotor activity and possesses anxiolytic properties. In addition, responses of 5-dpf zebrafish larvae to visual stimuli were not modified by DPH treatment.
二苯乙内酰脲（DPH）的抗癫痫作用已在动物研究和临床研究中得到证实，而关于该药物对基本行为和焦虑相关行为的影响却知之甚少。为了深入了解DPH的神经活性并在临床上合理使用DPH，有必要研究该药物的神经行为效应。在本研究中，探讨了在不同光照条件下DPH对受精后5天（dpf）斑马鱼幼体运动活性和趋触性的影响。还研究了DPH对斑马鱼幼体对视觉刺激（从亮到暗的突然光照转变）反应的影响。在光照或黑暗条件下，暴露于高浓度的DPH会导致运动活性和趋触性降低，而低剂量的DPH处理则增强了运动活性。此外，突然的光照转变会导致运动活性显著增加，并且这种现象不会因DPH处理而改变。我们的结果表明，DPH对运动活性具有潜在的刺激和抑制作用，并具有抗焦虑特性。此外，DPH处理不会改变5 dpf斑马鱼幼体对视觉刺激的反应。
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