Li Qiang, Lin Jia, Zhang Yinglan, Liu Xiuyun, Chen Xiao Qian, Xu Ming-Qing, He Lin, Li Sheng, Guo Ning
Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, China,
Psychopharmacology (Berl). 2015 Jan;232(1):197-208. doi: 10.1007/s00213-014-3656-5. Epub 2014 Jun 25.
Yohimbine demonstrated both anxiogenic and anxiolytic properties under different conditions in rodents. Few studies were conducted on zebrafish, a newly emerged vertebrate model organism. Zebrafish larvae are particularly suitable for high-throughput screening of drug effects.
The aim of this study was to elucidate the effect of yohimbine on the anxiety-related behaviors of zebrafish larvae.
AB strain zebrafish larvae at both 5 and 7 days postfertilization (dpf) were treated with different concentrations of yohimbine. General locomotor activities and thigmotaxis behavior were analyzed under continuous illumination, which represented normal condition, or under alternating light-dark challenges, which represented stressful environment.
Under continuous illumination, the 5-dpf larvae demonstrated increased swimming distances at low yohimbine concentrations, whereas the 7-dpf larvae demonstrated progressively decreased swimming distances with increases in yohimbine concentration. Low concentrations of yohimbine reduced thigmotaxis of the larvae, while high concentrations of yohimbine increased it. During the dark period of the light-dark challenge phase, low concentrations of yohimbine increased swimming distances of the larvae at both 5 and 7 dpf, while high concentrations of yohimbine decreased it. Yohimbine induced increased thigmotaxis in both 5- and 7-dpf larvae during the dark period.
Under normal condition (lights on), low doses of yohimbine were anxiolytic in both the 5- and 7-dpf larvae, whereas high doses of yohimbine were anxiogenic only in the 7-dpf larvae. Under mildly stressful condition (lights off), yohimbine treatment demonstrated dose-dependent effects to potentiate anxiety-related behaviors in both the 5- and 7-dpf larvae, although the significant dose varied with age.
育亨宾在啮齿动物的不同条件下表现出致焦虑和抗焦虑特性。针对斑马鱼这一新兴的脊椎动物模式生物开展的研究较少。斑马鱼幼体特别适合用于药物效应的高通量筛选。
本研究旨在阐明育亨宾对斑马鱼幼体焦虑相关行为的影响。
对受精后5天和7天（dpf）的AB品系斑马鱼幼体用不同浓度的育亨宾进行处理。在持续光照（代表正常条件）或明暗交替刺激（代表应激环境）下分析一般运动活动和趋触性。
在持续光照下，5 dpf的幼体在低育亨宾浓度时游泳距离增加，而7 dpf的幼体随着育亨宾浓度增加游泳距离逐渐减少。低浓度育亨宾降低了幼体的趋触性，而高浓度育亨宾则增加了趋触性。在明暗刺激阶段的黑暗期，低浓度育亨宾增加了5 dpf和7 dpf幼体的游泳距离，而高浓度育亨宾则降低了游泳距离。育亨宾在黑暗期使5 dpf和7 dpf幼体的趋触性均增加。
在正常条件下（光照时），低剂量育亨宾对5 dpf和7 dpf幼体均具有抗焦虑作用，而高剂量育亨宾仅对7 dpf幼体有致焦虑作用。在轻度应激条件下（光照关闭），育亨宾处理对5 dpf和7 dpf幼体的焦虑相关行为均表现出剂量依赖性增强作用，尽管显著剂量随年龄而异。
