Peng Xiaolan, Lin Jia, Zhu Yingdong, Liu Xiuyun, Zhang Yinglan, Ji Yongxia, Yang Xue, Zhang Yan, Guo Ning, Li Qiang
Translational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect, Institute of Pediatrics, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
Key Laboratory of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025, China.
Pharmacol Biochem Behav. 2016 Jun;145:55-65. doi: 10.1016/j.pbb.2016.03.010. Epub 2016 Mar 24.
Pentylenetetrazole (PTZ), γ-aminobutyrate (GABA) antagonist, is a convulsant drug, known to induce anxiety and seizures in zebrafish. Changes in the mobility of zebrafish under light-dark transitions reflect anxiety level, serving as a useful behavioral readout. The effects of PTZ treatment have yet to be assayed in this manner. Zebrafish larvae (AB strain) at both 5dpf (days post-fertilization) and 7dpf were treated with different concentrations of PTZ. General locomotor activity and thigmotaxis were analyzed under continuous illumination (normal conditions) or alternating light-dark cycles (stressful conditions). Zebrafish larvae of 5dpf and 7dpf exhibited different sensitivities to PTZ. Anxiety level, measured in terms of response to illumination transitions under the influence of PTZ, demonstrated contrasting tendencies. Dark-light transitions dramatically increased the locomotor activity of zebrafish larvae receiving 8mM PTZ which was indicative of anxiety. This study suggests that PTZ increases the susceptibility by activating the neuron, which perhaps makes light change easier to influence the anxiety level of larvae. We provide useful evidence for putative anti-anxiety drug screening.
戊四氮（PTZ）是一种γ-氨基丁酸（GABA）拮抗剂，是一种惊厥药物，已知可在斑马鱼中诱发焦虑和癫痫发作。斑马鱼在明暗转换下的活动变化反映了焦虑水平，可作为一种有用的行为指标。PTZ治疗的效果尚未以这种方式进行测定。对受精后5天（5dpf）和7天（7dpf）的斑马鱼幼虫（AB品系）用不同浓度的PTZ进行处理。在持续光照（正常条件）或明暗交替循环（应激条件）下分析一般运动活性和趋触性。5dpf和7dpf的斑马鱼幼虫对PTZ表现出不同的敏感性。在PTZ影响下，根据对光照转换的反应来衡量的焦虑水平呈现出相反的趋势。明暗转换显著增加了接受8mM PTZ的斑马鱼幼虫的运动活性，这表明存在焦虑。这项研究表明，PTZ通过激活神经元增加了易感性，这可能使光照变化更容易影响幼虫的焦虑水平。我们为推定的抗焦虑药物筛选提供了有用的证据。
