在马来西亚埃及伊蚊的实验性垂直传播中检测到持续性基孔肯雅病毒RNA，但未检测到传染性病毒。
Detection of Persistent Chikungunya Virus RNA but not Infectious Virus in Experimental Vertical Transmission in Aedes aegypti from Malaysia.
作者信息
Wong Hui Vern, Vythilingam Indra, Sulaiman Wan Yusof Wan, Lulla Aleksei, Merits Andres, Chan Yoke Fun, Sam I-Ching
机构信息
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Institute of Technology, University of Tartu, Nooruse 1, 50411, Tartu, Estonia.
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Institute of Technology, University of Tartu, Nooruse 1, 50411, Tartu, Estonia
出版信息
Am J Trop Med Hyg. 2016 Jan;94(1):182-6. doi: 10.4269/ajtmh.15-0318. Epub 2015 Nov 23.
Vertical transmission may contribute to the maintenance of arthropod-borne viruses, but its existence in chikungunya virus (CHIKV) is unclear. Experimental vertical transmission of infectious clones of CHIKV in Aedes aegypti mosquitoes from Malaysia was investigated. Eggs and adult progeny from the second gonotrophic cycles of infected parental mosquitoes were tested. Using polymerase chain reaction (PCR), 56.3% of pooled eggs and 10% of adult progeny had detectable CHIKV RNA, but no samples had detectable infectious virus by plaque assay. Transfected CHIKV RNA from PCR-positive eggs did not yield infectious virus in BHK-21 cells. Thus, vertical transmission of viable CHIKV was not demonstrated. Noninfectious CHIKV RNA persists in eggs and progeny of infected Ae. aegypti, but the mechanism and significance are unknown. There is insufficient evidence to conclude that vertical transmission exists in CHIKV, as positive results reported in previous studies were almost exclusively based only on viral RNA detection.
垂直传播可能有助于节肢动物传播病毒的维持，但基孔肯雅病毒（CHIKV）中是否存在垂直传播尚不清楚。对来自马来西亚的埃及伊蚊中CHIKV感染性克隆的实验性垂直传播进行了研究。对受感染亲代蚊子第二个生殖营养周期的卵和成年子代进行了检测。使用聚合酶链反应（PCR），56.3%的混合卵和10%的成年子代有可检测到的CHIKV RNA，但通过空斑试验没有样本检测到有感染性的病毒。来自PCR阳性卵的转染CHIKV RNA在BHK-21细胞中未产生有感染性的病毒。因此，未证实有活力的CHIKV的垂直传播。非感染性CHIKV RNA存在于受感染埃及伊蚊的卵和子代中，但其机制和意义尚不清楚。没有足够的证据得出CHIKV存在垂直传播的结论，因为先前研究中报道的阳性结果几乎完全仅基于病毒RNA检测。
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