Laganà Alessandro, Veneziano Dario, Spata Tyler, Tang Richard, Zhu Hua, Mohler Peter J, Kilic Ahmet
Department of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, United States of America.
Department of Clinical and Molecular Biomedicine, University of Catania, Catania, Italy.
PLoS One. 2015 Nov 24;10(11):e0143313. doi: 10.1371/journal.pone.0143313. eCollection 2015.
MicroRNAs (miRNAs or miRs) are small regulatory RNAs crucial for modulation of signaling pathways in multiple organs. While the link between miRNAs and heart disease has grown more readily apparent over the past three years, these data are primarily limited to small animal models or cell-based systems. Here, we performed a high-throughput RNA sequencing (RNAseq) analysis of left ventricle and other tissue from a pre-clinical ovine model. We identified 172 novel miRNA precursors encoding a total of 264 mature miRNAs. Notably, 84 precursors were detected in both the left ventricle and other tissues. However, 10 precursors, encoding 11 mature sequences, were specific to the left ventricle. Moreover, the total 168 novel miRNA precursors included 22 non-conserved ovine-specific sequences. Our data identify and characterize novel miRNAs in the left ventricle of sheep, providing fundamental new information for our understanding of protein regulation in heart and other tissues.
微小RNA（miRNA或miR）是一类小型调节性RNA，对多个器官中信号通路的调节至关重要。在过去三年中，miRNA与心脏病之间的联系愈发明显，但这些数据主要局限于小型动物模型或基于细胞的系统。在此，我们对临床前绵羊模型的左心室和其他组织进行了高通量RNA测序（RNAseq）分析。我们鉴定出172个新的miRNA前体，共编码264个成熟miRNA。值得注意的是，在左心室和其他组织中均检测到84个前体。然而，有10个编码11个成熟序列的前体是左心室特有的。此外，总共168个新的miRNA前体包括22个非保守的绵羊特异性序列。我们的数据鉴定并表征了绵羊左心室中的新miRNA，为我们理解心脏和其他组织中的蛋白质调节提供了重要的新信息。
