Chen Shih-Cheng, Telinius Niklas, Lin Han-Tse, Huang Min-Chang, Lin Chien-Chen, Chou Cheng-Hung, Hjortdal Jesper
Aeon Astron Europe B.V., Leiden, The Netherlands.
Department of Ophthalmology, Aarhus University Hospital, Aarhus, Denmark.
PLoS One. 2015 Nov 24;10(11):e0143511. doi: 10.1371/journal.pone.0143511. eCollection 2015.
The aim of this study was to test the use of BioCornea, a fish scale-derived collagen matrix for sealing full-thickness corneal perforations in mini-pigs. Two series of experiments were carried out in 8 Lan-Yu and 3 Göttingen mini-pigs, respectively. A 2mm central full thickness corneal perforation was made with surgical scissors and 2mm trephines. The perforations were sealed immediately by suturing BioCornea to the wounded cornea. The conditions of each patched cornea were followed-up daily for 3 or 4 days. Status of operated eyes was assessed with slit lamp examination or optical coherence tomography (OCT). Animals were sacrificed after the study period and the corneas operated were fixated for histological examination. Both OCT imaging and handheld slit lamp observations indicated that a stable ocular integrity of the perforated corneas was maintained, showing no leakage of aqueous humor, normal depth of anterior chamber and only mild swelling of the wounded cornea. Hematoxylin and eosin staining of the patched cornea showed no epithelial ingrowths to the perforated wounds and no severe leucocyte infiltration of the stroma. The fish scale-derived BioCornea is capable to seal full-thickness corneal perforation and stabilize the integrity of ocular anterior chamber in pre-clinic mini-pig models. BioCornea seems to be a safe and effective alternative for emergency treatment of corneal perforations.
本研究的目的是测试BioCornea的用途，BioCornea是一种源自鱼鳞的胶原蛋白基质，用于封闭小型猪的全层角膜穿孔。分别在8只蓝玉小型猪和3只哥廷根小型猪身上进行了两组实验。用手术剪刀和2毫米环钻制作一个2毫米的中央全层角膜穿孔。通过将BioCornea缝合到受伤的角膜上立即封闭穿孔。每天对每个修补的角膜情况进行3或4天的随访。用裂隙灯检查或光学相干断层扫描（OCT）评估手术眼的状况。在研究期结束后处死动物，并将手术的角膜固定用于组织学检查。OCT成像和手持裂隙灯观察均表明，穿孔角膜的眼内完整性得以维持，未见房水渗漏，前房深度正常，受伤角膜仅轻度肿胀。修补角膜的苏木精和伊红染色显示，穿孔伤口无上皮内生，基质无严重白细胞浸润。源自鱼鳞的BioCornea能够封闭全层角膜穿孔并稳定临床前小型猪模型眼前房的完整性。BioCornea似乎是角膜穿孔紧急治疗的一种安全有效的替代方法。
