Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran; Research Institute of Sciences and New Technology, Mashhad, Iran.
Biosens Bioelectron. 2016 Apr 15;78:80-86. doi: 10.1016/j.bios.2015.11.028. Epub 2015 Nov 12.
Analytical methods for detection and quantitation of chloramphenicol in blood serum and foodstuffs arse highly in demand. In this study, a colorimetric sandwich aptamer-based sensor (aptasensor) was fabricated for sensitive and selective detection of chloramphenicol, based on an indirect competitive enzyme-free assay using gold nanoparticles (AuNPs), biotin and streptavidin. The designed aptasensor acquires characteristics of AuNPs, including large surface area and unique optical properties, and strong interaction of biotin with streptavidin. In the absence of chloramphenicol, the sandwich structure of aptasensor forms, leading to the observation of sharp red color. In the presence of target, functionalized AuNPs could not bind to 96-well plates, resulting in a faint red color. The fabricated colorimetric aptasensor exhibited high selectivity toward chloramphenicol with a limit of detection as low as 451 pM. Moreover, the developed colorimetric aptasensor was successfully used to detect chloramphenicol in milk and serum with LODs of 697 and 601 pM, respectively.
用于检测和定量血清和食品中氯霉素的分析方法需求量很大。本研究基于间接竞争酶免分析，利用金纳米粒子（AuNPs）、生物素和链霉亲和素，构建了一种基于比色适体传感器（aptasensor）用于灵敏和选择性检测氯霉素。设计的适体传感器具有 AuNPs 的特性，包括大的表面积和独特的光学性质，以及生物素与链霉亲和素的强相互作用。在没有氯霉素的情况下，适体传感器形成三明治结构，导致观察到尖锐的红色。在存在目标物的情况下，功能化的 AuNPs 不能与 96 孔板结合，导致红色变浅。所制备的比色适体传感器对氯霉素具有很高的选择性，检测限低至 451 pM。此外，该比色适体传感器成功用于检测牛奶和血清中的氯霉素，检测限分别为 697 和 601 pM。
