Xie Yuqi, Ma Lu, Ling Shaoming, Ouyang Huixiang, Liang Aihui, Jiang Zhiliang
Key Laboratory of Regional Ecological Environment Analysis and Pollution Control in Western Guangxi (Baise University), Education Department of Guangxi Zhuang Autonomous Region, College of Chemistry and Environment Engineering, Baise University, Baise 533000, China.
Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
Nanomaterials (Basel). 2022 Apr 17;12(8):1374. doi: 10.3390/nano12081374.
Carbon dots (CDs) can be prepared from various organic (abundant) compounds that are rich in surfaces with -OH, -COOH, and -NH groups. Therefore, CDs exhibit good biocompatibility and electron transfer ability, allowing flexible surface modification and accelerated electron transfer during catalysis. Herein, CDs were prepared using a hydrothermal method with fructose, saccharose, and citric acid as C sources and urea as an N dopant. The as-prepared CDs were used to catalyze AgNO-trisodium citrate (TSC) to produce Ag nanoparticles (AgNPs). The surface-enhanced Raman scattering (SERS) intensity increased with the increasing CDs concentration with Victoria blue B (VBB) as a signal molecule. The CDs exhibited a strong catalytic activity, with the highest activity shown by fructose-based CDs. After N doping, catalytic performance improved; with the passivation of a wrapped aptamer, the electron transfer was effectively disrupted (retarded). This resulted in the inhibition of the reaction and a decrease in the SERS intensity. When bisphenol A (BPA) was added, it specifically bound to the aptamer and CDs were released, recovering catalytical activity. The SERS intensity increased with BPA over the concentration range of 0.33-66.67 nmol/L. Thus, the aptamer-adjusted nanocatalytic SERS method can be applied for BPA detection.
碳点（CDs）可由各种富含 -OH、-COOH 和 -NH 基团的有机（丰富）化合物制备而成。因此，碳点具有良好的生物相容性和电子转移能力，能够实现灵活的表面修饰，并在催化过程中加速电子转移。在此，以果糖、蔗糖和柠檬酸为碳源、尿素为氮掺杂剂，采用水热法制备了碳点。将制备的碳点用于催化硝酸银 - 柠檬酸钠（TSC）生成银纳米颗粒（AgNPs）。以维多利亚蓝 B（VBB）为信号分子时，表面增强拉曼散射（SERS）强度随碳点浓度的增加而增强。碳点表现出较强的催化活性，其中基于果糖的碳点活性最高。氮掺杂后，催化性能得到改善；随着包裹的适配体的钝化，电子转移被有效破坏（延迟）。这导致反应受到抑制，SERS 强度降低。当加入双酚 A（BPA）时，它特异性地与适配体结合，碳点被释放，催化活性得以恢复。在 0.33 - 66.67 nmol/L 的浓度范围内，SERS 强度随双酚 A 的增加而增强。因此，适配体调节的纳米催化 SERS 方法可用于双酚 A 的检测。
