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基于ColIb-P9的转座子供体质粒，用于恶臭假单胞菌和多种其他革兰氏阴性菌。

Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other gram negative bacteria.

作者信息

Boulnois G J, Varley J M, Sharpe G S, Franklin F C

出版信息

Mol Gen Genet. 1985;200(1):65-7. doi: 10.1007/BF00383313.

DOI:10.1007/BF00383313
PMID:2993815
Abstract

The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria.

摘要

描述了携带转座子Tn5的ColIb质粒衍生物pLG221的特性。该质粒可用于通过从大肠杆菌进行接合作用，将Tn5导入超出ColIb宿主范围的多种革兰氏阴性细菌中。质粒pLG221以及携带Tn10的类似质粒pLG223，作为转座子介导的诱变载体，在多种革兰氏阴性细菌中可能具有广泛用途。

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Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other gram negative bacteria.基于ColIb-P9的转座子供体质粒，用于恶臭假单胞菌和多种其他革兰氏阴性菌。
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2
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PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.通过苯酚处理制备转化脱氧核糖核酸
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Host range of conjugation and replication functions of the Escherichia coli sex plasmid Flac. Comparison with the broad host-range plasmid RK2.大肠杆菌性质粒Flac的接合与复制功能的宿主范围。与广宿主范围质粒RK2的比较。
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Molecular mechanism of Ti plasmid mobilization by R plasmids: isolation of Ti plasmids with transposon-insertions in Agrobacterium tumefaciens.R质粒介导Ti质粒转移的分子机制：根癌土壤杆菌中含转座子插入Ti质粒的分离
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