Bonamy C, Guyonvarch A, Reyes O, David F, Leblon G
Université Paris-Sud, Centre d'Orsay, Institut de Génétique et Microbiologie, France.
FEMS Microbiol Lett. 1990 Jan 1;54(1-3):263-9. doi: 10.1016/0378-1097(90)90294-z.
Plasmid DNA was efficiently electro-transformed into intact cells of nine Corynebacteria strains belonging to Brevibacterium lactofermentum, Brevibacterium flavum, Corynebacterium glutamicum and Corynebacterium melassecola. Relationships were explored between transformation efficiency and parameters such as electric field strength and pulse length, DNA concentration, physiological state and concentration of the cells. In optimal conditions, more than 10(7) transformants per microgram of DNA could be obtained. Electro-transformation with plasmid DNA isolated from different sources indicates that DNA modification may play a role in transformation efficiency.
质粒DNA被高效地电转化到属于乳酸发酵短杆菌、黄色短杆菌、谷氨酸棒杆菌和蜜二糖棒杆菌的9种棒状杆菌菌株的完整细胞中。研究了转化效率与电场强度、脉冲长度、DNA浓度、细胞生理状态和细胞浓度等参数之间的关系。在最佳条件下，每微克DNA可获得超过10^7个转化体。用从不同来源分离的质粒DNA进行电转化表明，DNA修饰可能在转化效率中起作用。
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