[菊欧文氏菌ENA49的recA基因]
[recA-genes of Erwinia chrysanthemi ENA49].
作者信息
Prokulevich V A, Fomichev Iu K
出版信息
Mol Gen Mikrobiol Virusol. 1989 Jan(1):40-5.
PMID:2659980
Abstract
The recA gene of Erwinia chrysanthemi ENA49 has been cloned in vivo in Escherichia coli K12, recA13 cells using the plasmid pULB113. On the basis or DNA repair and recombination deficiencies complementation, of restoration of the inducible "SOS"-response functions the functional identity of the cloned gene with the recA gene was concluded. The recA gene was localized in the 18th min region of the chromosomal genetical map of Erwinia chrysanthemi ENA49 between the genes proA and pheA.
摘要
菊欧文氏菌ENA49的recA基因已利用质粒pULB113在大肠杆菌K12的recA13细胞中进行了体内克隆。基于DNA修复和重组缺陷互补，以及诱导型“SOS”反应功能的恢复，得出克隆基因与recA基因功能相同的结论。recA基因定位于菊欧文氏菌ENA49染色体遗传图谱的第18分钟区域，位于proA基因和pheA基因之间。
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