Sun Y, Li Q, Wang G, Zhu D, Chen J, Li P
Wuhan Aquaculture Science Research Institute, Wuhan, China.
Wuhan Aquaculture Science Research Institute, Wuhan, China
Genet Mol Res. 2015 Nov 13;14(4):14286-90. doi: 10.4238/2015.November.13.12.
Twenty-eight polymorphic microsatellite markers were developed from the transcriptome of Ancherythoculter nigrocauda. These loci were used to characterize the genotypes of 48 individuals. The observed number of alleles per locus ranged from 5 to 11, with an average of 7.7. Expected and observed heterozygosities ranged from 0.437 to 0.978 and from 0.373 to 1.000, respectively. Four of these polymorphic microsatellite loci (HWB14, HWB18, HWB24, and HWB30) deviated significantly from the Hardy-Weinberg equilibrium after use of the sequential Bonferroni correction (P < 0.05). Twenty of the 28 loci could be successfully amplified in Culter alburnus. These novel markers will be useful for germplasm resource conservation and management of A. nigrocauda and C. alburnus.
从黑尾鲌转录组中开发出28个多态性微卫星标记。这些位点用于鉴定48个个体的基因型。每个位点观察到的等位基因数范围为5至11，平均为7.7。期望杂合度和观察到的杂合度分别为0.437至0.978和0.373至1.000。在使用顺序邦费罗尼校正后，这些多态性微卫星位点中的四个（HWB14、HWB18、HWB24和HWB30）显著偏离哈迪-温伯格平衡（P < 0.05）。28个位点中的20个可以在翘嘴鲌中成功扩增。这些新标记将有助于黑尾鲌和翘嘴鲌的种质资源保护和管理。
