从麦穗鱼（Pseudorasbora parva）中分离并鉴定19个多态性微卫星位点。

Isolation and characterization of 19 polymorphic microsatellite loci from the topmouth gudgeon, Pseudorasbora parva.

作者信息

Zeng C, Gul Y, Yang K, Cui L, Wang W-M, Gao Z-X

机构信息

Key Lab of Agricultural Animal Genetics, College of Fishery, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China.

出版信息

Genet Mol Res. 2011;10(3):1696-700. doi: 10.4238/vol10-3gmr1477.

DOI:10.4238/vol10-3gmr1477

PMID:21863563

Abstract

The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ezhou, Hubei province of China. The loci had 5 to 11 alleles, with a mean of 7.7 at each locus; 11 loci conformed to Hardy-Weinberg equilibrium. The expected and observed heterozygosities ranged from 0.237 to 0.973 and from 0.647 to 0.914, respectively. All microsatellite loci were in linkage equilibrium. These microsatellite markers are potentially useful for the assessment of population genetic structure during invasion and dispersal of P. parva in new habitats.

摘要

麦穗鱼（Pseudorasbora parva）被认为是亚洲以外许多国家中最具入侵性的鱼类之一。我们从麦穗鱼中分离并鉴定了19个微卫星位点。在中国湖北省鄂州市的一个野生种群中采集了40尾麦穗鱼样本，对这19个位点的多态性进行了检测。这些位点有5至11个等位基因，每个位点平均有7.7个等位基因；11个位点符合哈迪-温伯格平衡。预期杂合度和观察到的杂合度分别在0.237至0.973和0.647至0.914之间。所有微卫星位点均处于连锁平衡状态。这些微卫星标记对于评估麦穗鱼在新栖息地入侵和扩散过程中的种群遗传结构可能具有重要作用。