基于高通量测序的圆口铜鱼 20 个多态微卫星位点的分离与特征分析。

Isolation and characterization of 20 polymorphic microsatellites loci for Xenocypris davidi based on high-throughput sequencing.

机构信息

Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province, Zhejiang Research Center of East China Sea Fishery Research Institute, Zhejiang Institute of Freshwater Fisheries, 999th South Hangchangqiao Road, Wuxing District, Huzhou, 313000, China.

Jinhua Fisheries Technology Extension Station, Jinhua, 321000, China.

出版信息

Mol Biol Rep. 2020 Oct;47(10):8305-8310. doi: 10.1007/s11033-020-05834-4. Epub 2020 Sep 24.

DOI:10.1007/s11033-020-05834-4

PMID:32974841

Abstract

Xenocypris davidi is one of the most economically important freshwater fish in China. However, few molecular markers have been reported for this species, impeding in-depth population genetic, dispersal, and gene flow studies. In the present study, a batch of novel polymorphic microsatellites from the genome of X. davidi were isolated and characterized using high-throughput sequencing. A total of 20 microsatellite markers were isolated. Analysis of 33 individuals revealed an average of 7.35 alleles per locus, ranging from 3 to 18. The observed and expected heterozygosities ranged from 0.3 to 1 and from 0.426 to 0.93, respectively. Only one tested locus significantly deviated from Hardy-Weinberg equilibrium. 18 microsatellite loci were highly polymorphic (PIC > 0.5). These newly isolated microsatellite markers would be useful to study the population genetics and stock management of X. davidi.

摘要

黄河雅罗鱼是中国最重要的淡水经济鱼类之一。然而，目前针对该物种的分子标记数量较少，这阻碍了对其种群遗传、扩散和基因流的深入研究。本研究采用高通量测序技术从黄河雅罗鱼基因组中分离并鉴定了一批新的多态性微卫星标记。共分离出 20 个微卫星标记，对 33 个个体的分析表明，每个位点的平均等位基因数为 7.35，范围为 3-18。观测和预期杂合度分别为 0.3-1 和 0.426-0.93。仅一个测试位点显著偏离哈迪-温伯格平衡。18 个微卫星位点高度多态（PIC＞0.5）。这些新分离的微卫星标记将有助于研究黄河雅罗鱼的种群遗传和种群管理。