Kabiri Mahboubeh, Oraee-Yazdani Saeed, Dodel Masumeh, Hanaee-Ahvaz Hana, Soudi Sara, Seyedjafari Ehsan, Salehi Mohammad, Soleimani Masoud
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran ; Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, Iran ; Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, Iran.
Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, Iran ; Functional Neurosurgery Research Center, Shohada Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
EXCLI J. 2015 Jul 27;14:851-60. doi: 10.17179/excli2015-282. eCollection 2015.
The purpose of this study was to fabricate a conductive aligned nanofibrous substrate and evaluate its suitability and cytocompatibility with neural cells for nerve tissue engineering purposes. In order to reach these goals, we first used electrospinning to fabricate single-walled carbon-nanotube (SWCNT) incorporated poly(L-lactic acid) (PLLA) nanofibrous scaffolds and then assessed its cytocompatibility with olfactory ensheathing glial cells (OEC). The plasma treated scaffolds were characterized using scanning electron microscopy and water contact angle. OECs were isolated from olfactory bulb of GFP Sprague-Dawley rats and characterized using OEC specific markers via immunocytochemistry and flow cytometery. The cytocompatibility of the conductive aligned nano-featured scaffold was assessed using microscopy and MTT assay. We indicate that doping of PLLA polymer with SWCNT can augment the aligned nanosized substrate with conductivity, making it favorable for nerve tissue engineering. Our results demonstrated that SWCNT/PLLA composite scaffold promote the adhesion, growth, survival and proliferation of OEC. Regarding the ideal physical, topographical and electrical properties of the scaffold and the neurotrophic and migratory features of the OECs, we suggest this scaffold and the cell/scaffold construct as a promising platform for cell delivery to neural defects in nerve tissue engineering approaches.
本研究的目的是制备一种导电排列的纳米纤维基质，并评估其对神经组织工程中神经细胞的适用性和细胞相容性。为了实现这些目标，我们首先采用静电纺丝法制备了掺入单壁碳纳米管（SWCNT）的聚（L-乳酸）（PLLA）纳米纤维支架，然后评估其与嗅鞘胶质细胞（OEC）的细胞相容性。采用扫描电子显微镜和水接触角对经等离子体处理的支架进行表征。从绿色荧光蛋白Sprague-Dawley大鼠的嗅球中分离出OEC，并通过免疫细胞化学和流式细胞术使用OEC特异性标记物对其进行表征。使用显微镜和MTT法评估导电排列的纳米结构支架的细胞相容性。我们表明，用SWCNT掺杂PLLA聚合物可以增强排列的纳米尺寸基质的导电性，使其有利于神经组织工程。我们的结果表明，SWCNT/PLLA复合支架促进了OEC的黏附、生长、存活和增殖。鉴于支架理想的物理、拓扑和电学性质以及OEC的神经营养和迁移特性，我们建议这种支架以及细胞/支架构建体作为神经组织工程方法中向神经缺损部位递送细胞的一个有前景的平台。
