Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Biotechnol Lett. 2012 Jul;34(7):1357-65. doi: 10.1007/s10529-012-0889-4. Epub 2012 Apr 3.
Nerve tissue engineering requires suitable precursor cells as well as the necessary biochemical and physical cues to guide neurite extension and tissue development. An ideal scaffold for neural regeneration would be both fibrous and electrically conductive. We have contrasted the growth and neural differentiation of mouse embryonic stem cells on three different aligned nanofiber scaffolds composed of poly L: -lactic acid supplemented with either single- or multi-walled carbon-nanotubes. The addition of the nanotubes conferred conductivity to the nanofibers and promoted mESC neural differentiation as evidenced by an increased mature neuronal markers expression. We propose that the conductive scaffold could be a useful tool for the generation of neural tissue mimics in vitro and potentially as a scaffold for the repair of neural defects in vivo.
神经组织工程需要合适的前体细胞以及必要的生化和物理线索来引导轴突延伸和组织发育。理想的神经再生支架应该既具有纤维状又具有导电性。我们比较了三种不同的、由聚 L: -乳酸组成的取向纳米纤维支架上的小鼠胚胎干细胞的生长和神经分化，这些支架中添加了单壁或多壁碳纳米管。纳米管的添加赋予了纳米纤维导电性，并促进了 mESC 的神经分化，表现为成熟神经元标志物表达增加。我们提出，这种导电支架可能是体外生成神经组织模拟物的有用工具，并且可能作为体内神经缺陷修复的支架。
